alexa Clinical Significance of Beclin-1 Dependent Autophagy Induced by Imatinib in Chronic Myeloid Leukemia Patients

Journal of Mucosal Immunology Research
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Clinical Significance of Beclin-1 Dependent Autophagy Induced by Imatinib in Chronic Myeloid Leukemia Patients

Esmat A. El-Sharkawy1, Khalid M. Salah1, Amel Mahmoud Kamal Eldin1*, Ayman Gamil Ghobrial1, Walid M. Abdel-Hamid1, Eman Hassan2 and Rasha Y. Ali2

1Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt

2Clinical Pathology Department, Faculty of Medicine, Assiut University, Egypt

*Corresponding Author:
Amel Mahmoud Kamal Eldin
MD, PhD, Department of Clinical Pathology
Faculty of Medicine, Minia University, Minia, Egypt
Tel: +201019748497
E-mail: [email protected]

Received date: February 15, 2017; Accepted date: June 26, 2017; Published date: June 30, 2015

Citation: El-Sharkawy EA, Salah KM, Kamal Eldin AM, Ghobrial AG, Abdel-Hamid WM, et al. (2017) Clinical Significance of Beclin-1 Dependent Autophagy Induced by Imatinib in Chronic Myeloid Leukemia Patients. J Mucosal Immunol Res 1:103.

Copyright: © 2017 El-sharkawy EA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Background and objectives: The introduction of imatinib as one of the tyrosine kinase inhibitors (TKI) has revolutionized the treatment of chronic myelogenous leukemia (CML), representing the first target specific drug. Autophagy is a constitutive homeostatic mechanism for intracellular recycling and metabolic regulation. The role of autophagy in cancer is very complex, it is tumor suppression mechanism yet it enables tumor cell survival in stress. The aim of the present study was to assess autophagy induced by imatinib therapy through beclin-1 gene in CML patients, and to evaluate beclin-1 gene expression in relation to patients’ clinical and hematological response.

Subjects and methods: The present study was carried out on 50 subjects, 35 newly diagnosed CML patients who were classified into 2 groups: (Group I) including 17 CML patients and (Group II) including 18 CML patients in accelerated phase, and 15 apparently healthy subjects served as controls (Group III). All patients were treated with imatinib and followed up after six weeks of treatment. Each patient was subjected to complete history taking, clinical examination along with laboratory investigations in the form of liver & renal function tests, random blood glucose, ESR, CBC & examination of Leishman-stained peripheral blood and Bone marrow aspiration and examination of Leishman-stained smears. The diagnosis was confirmed by detection of BCR-ABL gene expression by quantitative RT-PCR. Beclin-1 gene expression in peripheral blood samples was assessed as well via quantitative RT-PCR for all subjects along with its reassessment in follow-up samples for all patients.

Results: All patients in the study have achieved complete clinical and hematological response after six weeks of the beginning of imatinib therapy along with a highly significant increase in beclin-1 gene expression levels after treatment, (P<0.001) than before treatment in both patients groups I & II. There was no significant difference between group I & group II, regarding beclin-1 gene expression results after treatment (p=0.631). There was a high statistically significant negative correlation between beclin-1 gene expression before treatment and BCR-ABL gene expression results (r=-0.844 & -0.822) in group I and II respectively.

Conclusions: We concluded that beclin-1 dependent autophagy is increased after the beginning of imatinib therapy indicating induction of autophagy in CML patients, along with complete clinical and hematological response.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version