Detection of SARS-CoV-2 in Saliva Using Tailed Amplicon Sequencing | OMICS International| Abstract
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Infect Dis Ther,
  • DOI: 10.4172/2332-0877.1000464

Detection of SARS-CoV-2 in Saliva Using Tailed Amplicon Sequencing

Aaron Garoutte1, Tasha M Santiago-Rodriguez1, Heather L Fehling2 and Rafal Iwasiow3*
1Diversigen, Houston, USA
2Clinical Reference Laboratory, Lenexa, USA
3DNA Genotek, Ottawa, Canada
*Corresponding Author : Rafal Iwasiow, DNA Genotek, Ottawa, Canada, Email: [email protected]

Received Date: Jun 08, 2021 / Accepted Date: Jun 22, 2021 / Published Date: Jun 29, 2021


The most recent virus from the Coronaviridae family infecting humans, SARS-CoV-2, has resulted in a global pandemic. As part of the surveillance efforts, SARS-CoV-2 genomes are increasingly being made publicly available. Methods that include both short- and long-read sequencing have been used to elucidate SARS-CoV-2 genomes; however, many of these untargeted approaches may require deeper sequencing for greater genome coverage. For this reason, sequence capture or amplicon-based approaches for SARS-CoV-2 genome sequencing have been developed. The present proof-of-concept study evaluated a modified sequence capture approach, namely, tailed amplicon sequencing, to determine SARS-CoV-2 near complete genome sequences from the saliva of infected individuals. Particularly, the suitability of saliva samples stored at room temperature using OMNIgene® •ORAL OME-505 was evaluated. The tailed amplicon sequencing approach poses the additional advantage of being a cost-effective method for library preparation. Different known SARS-CoV-2 variants were identified across the infected subjects, with an average of >99.4% genome coverage. This methodology also enabled robust genomic surveillance using phylogenetic analyses. The present study supports the suitability of saliva stored at room temperature using collection devices for SARS-CoV-2 variant detection. Importantly, the present study supports the use of tailed amplicon sequencing approaches as an alternative, cost-effective method for SARS-CoV-2 detection in saliva for genomic surveillance.

Keywords: Genomic surveillance; SARS-CoV-2; Sequence capture; Tailed amplicon sequencing

Citation: Garoutte A, Santiago-Rodriguez TM, Fehling HL, Iwasiow R (2021) Detection of SARS-CoV-2 in Saliva Using Tailed Amplicon Sequencing. J Infect Dis Ther 9:464. Doi: 10.4172/2332-0877.1000464

Copyright: © 2021 Garoutte A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Article Usage
  • Total views: 277
  • [From(publication date): 0-0 - Sep 28, 2021]
  • Breakdown by view type
  • HTML page views: 124
  • PDF downloads: 153