Development of Improved Sandwich Elisa for the In Vitro Detection of Inhibitors of the TNF-TNFR1 Interaction LC/MS
Jessica M Davis*
Department of Chemistry & Biochemistry, Fairfield University, BNW 315, USA
- *Corresponding Author:
- Jessica M Davis, Ph.D
Department of Chemistry & Biochemistry
Fairfield University, BNW 315, USA
Tel: 203-254- 4000 x2123
E-mail: [email protected]
Received date: November 04, 2011; Accepted date: December 22, 2011; Published date: December 24, 2011
Citation: Davis JM (2012) Development of Improved Sandwich Elisa for the In Vitro Detection of Inhibitors of the TNF-TNFR1 Interaction. J Anal Bioanal Tech 3:129. doi: 10.4172/2155-9872.1000129
Copyright: © 2012 Davis JM. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The over-expression of tumor necrosis factor alpha (TNF-alpha) has been associated with various diseases, particularly autoimmune diseases such as rheumatoid arthritis and Crohn’s disease. Some biologic therapies for these diseases specifically target this cytokine, sequestering it, and preventing its interaction with its 55 kD receptor (TNFR 1 ). While these therapies have proven the validity of this approach, they are large proteins that require intravenous administration. Small molecule inhibitors of this interaction are highly desirable and some have been developed. However, such inhibitors have not been reported to have successfully completed clinical trials. One prohibition to this approach is the current state of in vitro testing of such molecules. Assays have been developed that use radio labeled or biotin labeled TNF-alpha. In vivo methods exist, but are cost prohibitive to screening a large selection of molecules. Reported here-in is an inexpensive, highly robust, sensitive assay for inhibitors of the TNF- alpha interaction with TNFR 1 that does not require modification of TNF-alpha. The simplicity of this assay should allow for increased investigation into inhibiting this important interaction.