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Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells| Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

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  • Research Article   
  • J Bioremediat Biodegrad,

Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells

Aifeng Liu2, Qiang Wei1*, Jixin Chen2, Shuwei Gong3 and Ye Yuan4*
1Department of Mechanical Engineering, Hebei University of Technology, Tianjin 300193, China
2Department of Orthopaedic Surgery, University of Traditional Chinese Medicine, Tianjin 300193, China
3Department of Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
4Department of Tianjin Key Laboratory of Materials Laminating Fabrication and Interface Control Technology, Hebei University of Technology, Tianjin 300130, China
*Corresponding Author (s) : Dr. Qiang Wei, Department of Mechanical Engineering, Hebei University of Technology, Tianjin, China, Email: [email protected]
Dr. Ye Yuan, Department of Tianjin Key Laboratory of Materials Laminating Fabrication and Interface Control Technology, Hebei University of Technology, Tianjin 300130, China, Email: [email protected]

Received Date: Dec 01, 2021 / Accepted Date: Dec 15, 2021 / Published Date: Dec 22, 2021

Abstract

The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodeg-radability. Thus, the two-dimensional Graphene Oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture. In this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. It was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. The adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.

Keywords: Graphene oxide; Mesenchymal stem cell; Co-culture; Scaffolds; Knee osteoarthritis

Citation: Wei Q, Liu A, Chen J, Gong S, Yuan Y (2021) Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells. J Bioremediat Biodegrad 12:013.

Copyright: © 2021 Wei Q, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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