alexa In vitro Plant Regeneration System for Two Middle East Cultivars of Chickpea (Cicer arietinum L.)
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

In vitro Plant Regeneration System for Two Middle East Cultivars of Chickpea (Cicer arietinum L.)

Aline Kadri1,2*, Lamis Chalak3, Ahmad El Bitar2, Nancy Nicolas2, Souad Mroué2 and Ghislaine Grenier De March4
1Institut Polytechnique LaSalle Beauvais, France
2Lebanese Agricultural Research Institute (LARI), Lebanon
3Faculty of Agricultural Sciences, Lebanese University, Lebanon
4Champagne Ardenne Research & Innovation Agency (CARINNA), France
Corresponding Author : Kadri Aline
Lebanese Agricultural Research Institute (LARI)
Zahle P.O. Box 287-Lebanon
Tel: 961 8 900037
Fax: 961 8 900077
E-mail: [email protected]
Received February 13, 2014; Accepted March 22, 2014; Published March 24, 2014
Citation: Kadri A, Chalak L, El Bitar A, Nicolas N, Mroué S, Grenier De March G (2014) In vitro Plant Regeneration System for Two Middle East Cultivars of Chickpea (Cicer arietinum L.). Adv Crop Sci Tech 2: 125. doi: 10.4172/2329-8863.1000125
Copyright: © 2014 Kadri A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

This study surveyed a rapid, efficient and reproducible protocol for in vitro shoot regeneration and rooting of two Middle East chickpea cultivars (Balila and Wady). Organogenic ability of various explants (decapitated embryo axes, fragments of embryo axes, fragments of embryo axes with basal part of cotyledons, entire and half of cotyledons) was tested on MS media containing different concentrations and nature of cytokinins (BAP and zeatin) or compound with cytokinin-like activity (TDZ). The highest level of shoot regeneration was achieved in Balila genotype using embryo axes fragments including the basal part of cotyledon on medium with 0.25 mg/L BAP. Higher BAP concentrations than 0.25 mg/L for Balila and 0.5 mg/L for Wady caused a decrease of shoot regeneration. On MS media supplemented with zeatin (1, 3 and 5 mg/L) and thidiazuron (1, 3 and 5 mg/L), the highest level of shoot differentiation was obtained from calli derived from fragments of embryo axes. Organogenic ability of calli from Wady genotype was enhanced by increasing zeatin and TDZ concentrations, up to 5 mg/L. Conversely, a progressive decrease of shoot frequency for Balila and root development for both genotypes, were observed with increasing concentrations of BAP, zeatin and TDZ.

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