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Interaction Effects of 6-Benzylaminopurine and Kinetin on In vitro Shoot Multiplication of Two Sugarcane (Saccharum officinarum L.) Genotypes | OMICS International| Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
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  • Research Article   
  • Adv Crop Sci Tech 2014, Vol 2(4): 143
  • DOI: 10.4172/2329-8863.1000143

Interaction Effects of 6-Benzylaminopurine and Kinetin on In vitro Shoot Multiplication of Two Sugarcane (Saccharum officinarum L.) Genotypes

Dereje Shimelis1*, Kassahun Bantte2 and Tilaye Feyissa3
1Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, P. O. box 15, Wonji, Ethiopia
2College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia
3Science and Technology faculty, Addis Abeba University, Addis Abeba, Ethiopia
*Corresponding Author : Dereje Shimelis, Ethiopian Sugar Corporation, Research and Training Division, Variety Development Directorate, Biotechnology Research Team, Wonji Research Center, P. O. Box 15, Wonji, Ethiopia, Tel: +25191081644, Email: [email protected]

Received Date: Jul 30, 2014 / Accepted Date: Sep 27, 2014 / Published Date: Sep 29, 2014

Abstract

In Ethiopia, sugarcane is grown as an important cash and industrial crop. It is not an ideal crop for conventional breeding and it lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize the optimum concentration of 6-benzylaminopurine (0.0, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mgL-1) and Kinetin (0.0, 0.1, 0.5, and 1.0 mgL-1) combination for shoot multiplication of C86-12 and C86-56 genotypes in completely randomized design with 5x4x2 factorial treatment combinations arrangements. The analysis of variance showed that the interaction effects of BAP, kinetin and genotypes on the number of shoots per explant, number of leaves per shoot and average shoot length were highly significant (p<0.001). Murashige and Skoog (MS) medium supplemented with 1.5 mgL-1 BAP and 0.5 mgL-1 of Kin for B86-12; and 1.5 mgL-1 of BAP and 1.0 mgL-1 of Kin for C86-56 were found to be the optimum media for shoot multiplication. B86-12 showed 33.8 ± 0.837 number of shoots per explant with 13.04 ± 0.089 average number of leaves per shoot and 8.4 ± 0.008 cm shoot length whereas C86-56; 25.6 ± 0.548 number of shoots per explant with average number of leaves per shoot of 9.8 ± 0.447 and shoot length 8.65 ± 0.72 cm was obtained after 30 days of sub culturing. Thus, the optimized protocol can be used for rapid multiplication of the planting materials

Keywords: Sugarcane; BAP; Kinetin; Shoot multiplication

Citation: Shimelis D, Bantte K, Feyissa T (2014) Interaction Effects of 6-Benzylaminopurine and Kinetin on in vitro Shoot Multiplication of Two Sugarcane (Saccharum officinarum L.) Genotypes. Adv Crop Sci Tech 2:143. Doi: 10.4172/2329-8863.1000143

Copyright: © 2014 Shimelis D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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