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Maneb and Mancozeb Increase Amyloid β Precursor Protein Expression and Activate PKR | OMICS International| Abstract

Journal of Cell Science and Apoptosis
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  • Research Article   
  • J Cell Sci Apo 1: 110,

Maneb and Mancozeb Increase Amyloid β Precursor Protein Expression and Activate PKR

Shu-Yuan Cheng1,2*, Yessenia Lopez3 and Jazlene Montes1
1Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York 10019, USA
2Graduate Center, City University of New York, New York, NY 10016, USA
3Biophysics, Physiology, and Systems Biology Program, Cornell Graduate School of Medical Sciences, , New York, NY, 10065, USA
*Corresponding Author : Shu-Yuan Cheng, Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York 10019, USA, Tel: 646-557- 4637, Email: [email protected]

Received Date: Oct 24, 2017 / Accepted Date: Nov 16, 2017 / Published Date: Nov 26, 2017

Abstract

Background: Oligomers of amyloid β (Aβ) in the brain correlate with synapse loss and dementia. The fungicide maneb (MB) and mancozeb (MZ) have been shown to activate the transcription factor NF-κB. Activation of NF-κB signaling pathway could initiate the transcription of β-amyloid precursor protein (AβPP) leading to increase Aβ level. Studies showed the cells treated with Aβ42 have the elevated level of phosphorylated double-stranded RNA dependent protein kinase (PKR) which is found to deteriorate the neurons in AD brains.
Objective:
To reveal the effects of MB and MZ on AβPP and Aβ42 expressions and to elucidate the role of PKR in response to MB and MZ.
Methods: Western blot analysis and ELISA assay were conducted to investigate the expressions of AβPP and Aβ42 in PC12 cells treated with MB and MZ. The activation of PKR was determined by western blotting the level of phosphorylated PKR at Thr446 in SH-SY5Y cells treated with MB and MZ.
Results: MB and MZ increased AβPP and Aβ42 expressions in a dose-dependent manner. MB and MZ transiently activated PKR.
Conclusion: Studies showed that MB and MZ enhance parkinsonian toxin MPP+ cytotoxicity and trigger DNA damage. The results from this study further revealed that MB and MZ are associated with the increase of AβPP and Aβ42 expressions and elucidated that the PKR signaling pathway involves in MB and MZ induced cytotoxicity. This study provides the evidence about the relationship between fungicides and neurodegenerative diseases.

Keywords: Maneb; Mancozeb; AβPP; Aβ42; PKR

Citation: Cheng SY, Lopez Y, Montes J (2017) Maneb and Mancozeb Increase Amyloid β Precursor Protein Expression and Activate PKR. J Cell Sci Apo 1: 110.

Copyright: © 2017 Cheng SY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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