alexa Marker-Assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar "REDWOLAITA"| Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
  • Research Article   
  • Adv Crop Sci Tech 2019, Vol 7(1): 416
  • DOI: 10.4172/2329-8863.1000416

Marker-Assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar "REDWOLAITA"

Yayis Rezene1,5*, Kassahun Tesfaye2,3, Clare Mukankusi4 and Paul Gepts5
1Molecular Biotech Laboratory, Southern Agricultural Research Institute, Awassa, Ethiopia
2Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia
3Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Ethiopia
4International Centre for Tropical Agriculture (CIAT), Kampala, Uganda
5Department of Plant Sciences, University of California, MS 1 Shields Avenue, Davis, California, USA
*Corresponding Author : Yayis Rezene, Molecular Biotech Laboratory, Southern Agricultural Research Institute, PO Box 06 Awassa, Ethiopia, Tel: +251911744294, Email: [email protected]

Received Date: Nov 21, 2018 / Accepted Date: Jan 24, 2019 / Published Date: Feb 05, 2019


Angular leaf spot (ALS) caused by Pseudocercospora griseola and common bacterial blight (CBB) caused by Xanthomonas campestris pv phaseoli X. campestris pv. phaseoli var. fuscans are the most economically important diseases of common bean production in Ethiopia. This research was aimed at pyramiding the Phg-2 R gene for angular leaf spot resistance and two CBB major resistance quantitative trait loci (RQTLs) into the background of the most popular and susceptible common bean cultivar “REDWOLAITA” (RW). Marker-assisted Parallel Back Crossing (MAPBC) breeding scheme with three separate parallel backcrossing streams were adopted. This technique accelerated tracking three independent resistance loci linked to g796, SAP6 and SU91 genetic marker from two different donor parents into the RW recurrent parent. The donor parental line VAX-6 with known RQTLs for CBB and tagged by the SAP6 and SU91 genetic markers on linkage groups 10 and 8, respectively was used. The other donor parent MEX-54 with known Phg-2 R gene tagged by the g796 genetic marker at the linkage group 8 was used in the gene pyramiding program. After the BC4 generation, progenies that combined SAP6 and g796 genetic markers were created and selected from the BC4 inter-crossing of progenies. Then, further inter-crossing was made between selected progenies that combined the SAP6 and g796 genetic markers with selected progenies with the SU91 genetic marker. Finally, from this study we developed Monogenic Near Isogenic Lines (MNILs) with R genes tagged by the SAP6, g796, and SU91 molecular markers and Polygenic Near Isogenic Lines (PNILs) with different gene combination. The developed lines include MNILSAP6, MNILSU91 and MNILg796, PNILsSAP6/g796, PNILsSU91/g796, PNILsSAP6/SU, PNILsSAP6/g796/SAP6, with more than 97% genome recovered from the RW genetic background. Marker-assisted backcrossing facilitated selection of progenies that combined good agronomic traits with resistance loci were constructed from the RW common bean cultivar. The developed lines showed high level of disease resistance to the strains of CBB and ALS under the screening conditions. The developed lines will be evaluated under multiple environment under natural epidemics, before varietal release for wider production. We also recommend the developed NILs with RW back ground and known sources of R genes and good agronomic performance could be used as alternative donor parent for the future gene pyramiding program.

Keywords: Gene pyramiding; Parallel backcrossing; RQTLs; Inter-crossing; Isogenic lines

Citation: Rezene Y, Tesfaye K, Mukankusi C, Gepts P (2019) Marker-Assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar “REDWOLAITA”. Adv Crop Sci Tech 7: 416. Doi: 10.4172/2329-8863.1000416

Copyright: © 2019 Rezene Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Leave Your Message 24x7