alexa Optimization of EMS Mutagenesis on Petunia for TILLING | OMICS International
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

Optimization of EMS Mutagenesis on Petunia for TILLING

Jiang P*, Chen Y and Wilde HD
Horticulture Department, University of Georgia, Athens, Georgia, USA
Corresponding Author : Jiang P
Horticulture Department, University of Georgia
Athens, Georgia, USA 30602
Tel: 1-706-201-9609
E-mail: [email protected]
Received August 27, 2014; Accepted September 23, 2014; Published September 23, 2014
Citation: Jiang P, Chen Y, Wilde HD (2014) Optimization of EMS Mutagenesis on Petunia for Tilling, Ethiopia. Adv Crop Sci Tech 2:141. doi: 10.4172/2329-8863.1000141
Copyright: © 2014 Jiang P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The development of a chemically-mutagenized population of Petunia hybrida could enable the identification of
novel alleles for crop improvement. Conditions were determined for mutagenizing petunia with ethyl methanesulfonate (EMS), while minimizing deleterious effects on viability and fertility. A mutagenized population of the doubled haploid P. hybrida line ‘Mitchell Diploid’ was developed as a resource for TILLING. Three mutagenesis parameters were investigated in this study: the imbibition of seeds prior to EMS treatment, the EMS concentration, and EMS exposure time. Based on these results, the mutagenesis of 2000 petunia seeds for TILLING was conducted with a 12 hour imbibition followed by exposure to 0.1% EMS for 12 hours.

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