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  • Research Article   
  • Cell Mol Biol,
  • DOI: 10.4172/1165-158X.1000207

Osteopontin Isoforms b and c Mediate Prostate Cancer Cell Evasion

Akram Mirzaei1, Sina Rashedi2, Rahil Mashhadi1, Seyyed Mohammad Ghahestani3, Alireza Gorji1, Leonardo Olivia Reis4, Fatemeh Khatami1 and Seyed Mohammad Kazem Aghamir1*
1Urology Research Center,, Tehran University of Medical Sciences, Tehran, Iran
2School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3Children’s Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran
4UroScience and Department of Surgery (Urology), School of Medical Sciences, University of Campinas, Unicamp and Pontifical Catholic University of Campinas, PUCCampinas, Campinas, São Paulo, Brazil
*Corresponding Author : Seyed Mohammad Kazem Aghamir, Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran, Tel: + 982166348560, Email: mkaghamir@tums.ac.in

Received Date: Aug 10, 2021 / Accepted Date: Sep 09, 2021 / Published Date: Sep 16, 2021

Abstract

Background: Osteopontin (OPN), an extracellular structural protein, along VEGF are attributed to cancer progression and angiogenesis. Therefore, we aimed to evaluate the effect of Curcumin and anti-androgen drug, Flutamide (separately and in combination) on the expression of OPN isoforms and VEGF.

Methods: The human prostate cell lines were treated with different concentrations of Curcumin and prostate cancer conventional drug Flutamide alone and combined to find effective doses and IC50 values. Percentages of apoptotic cells were evaluated by Annexin/PI staining, and mRNA levels of OPN isoforms and VEGF gene expression were investigated by the real-time PCR method.

Results: Our data displayed that Flutamide (20 μM and 12 μM in PC3 and LNCaP cell lines, respectively), Curcumin (15 μM and 10 μM in PC3 and LNCaP cell lines, respectively), and also their combination with same above concentrations significantly increase the percentage of apoptotic cells with upregulation of tumor suppressor P53 gene, and downregulation of anti-apoptotic Bcl-2 gene. Also, it causes overexpression of OPN-b and OPN-c isoforms and VEGF gene in androgen-independent (PC3) and OPN-c isoform and VEGF in androgen-dependent (LNCaP) prostate

Conclusion: We show for the first time that prostate cancer cells probably evade Curcumin and Flutamide antiandrogenic effects via induction of OPN-b and OPN-c isoforms, which are recognized factors with the capacity of promoting cancer progression and angiogenesis. The correlation between androgen-independency and OPN-b isoforms needs further exploration.

Keywords: Prostate cancer; Curcumin; Flutamide; Osteopontin; VEGF; Angiogenesis; Resistance

Citation: Mirzaei A, Rashedi S, Mashhadi R, Ghahestani SM, Gorji A, et al. (2021) Osteopontin Isoforms b and c Mediate Prostate Cancer Cell Evasion. Cell Mol Biol 67: 207. Doi: 10.4172/1165-158X.1000207

Copyright: © 2021 Mirzaei A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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