ISSN: 2329-8863

Advances in Crop Science and Technology
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  • Research Article   
  • Adv Crop Sci Tech 2017, Vol 5(1): 252
  • DOI: 10.4172/2329-8863.1000252

Promoter Analysis of a Powdery Mildew Induced Vitis vinifera NAC Transcription Factor Gene

Solomon Abera Gebrie*
Ethiopian Institute of Agricultural Research, Holoeta Research Center, Holeta, Ethiopia
*Corresponding Author : Solomon Abera Gebrie, Ethiopian Institute of Agricultural Research, Holeta, Ethiopia, Tel: +251911568273, Email: solomonabera19@gmail.com

Received Date: Dec 27, 2016 / Accepted Date: Jan 09, 2017 / Published Date: Jan 16, 2017

Abstract

Grapevine is an economically important crop throughout the world. However commonly cultivated species Vitis vinifera is susceptible to powdery mildew fungi. The objective of this study was to analyze the promoter of VvNAC36 transcription factor gene, which was induced by powdery mildew infection, as it was shown by previous microarray results. Analysis of VvNAC36 transcription factor promoter allows identifying the important cis-elements, which are responsible for gene regulation during infection. Knowing that the responsible cis-element of the gene is crucial for further study, the role of this gene is in powdery mildew infected grapevine. The study was performed using circumspect methodology. To confirm the presence of transgene in transgenic plants the PCR and glufosinate total herbicide application was used. The transplanted transgenic seedlings were infected with Golovinomyces orontii. To detect the GUS reporter gene expression, histochemical GUS staining and spectrophotometric assays were applied in each deletion variant. Finally the promoter sequence was analyzed by the PLACE (a database for PLAntCisacting Elements) program for identifying cis-elements. The results of the study showed significant difference in mock treated and induced expression in transgenic plants transformed with VvNAC36 promoter segments of different lengths, fused to GUS reporter gene. In basal expression level, the deletion variants 1178 bp and 257 bp differed significantly. Powdery mildew infection significantly increased the expression in all promoter variants, except the two shortest fragments. Based on the promoter motif analysis, it can be concluded, that the GT1-box (GAAAAA) supposed to have crucial role in regulating of VvNAC36 transcription factor gene during powdery mildew infection.

Keywords: VvNAC36 transcription factor; Powdery mildew; Gene expression; Cis-elements

Citation: Gebrie SA (2017) Promoter Analysis of a Powdery Mildew Induced Vitis vinifera NAC Transcription Factor Gene. Adv Crop Sci Tech 5:252. Doi: 10.4172/2329-8863.1000252

Copyright: © 2017 Gebrie SA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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