Purification of Schizothorax Immunoglobulin Raised Against Metazoan Parasite: A Preliminary Study
- *Corresponding Author:
- Feroz A. Shah
Faculty of Fisheries
Shuhama, J&K, India
E-mail: [email protected]
Received Date: April 27, 2013; Accepted Date: May 20, 2013; Published Date: May 26, 2013
Citation: Shah FA, Mohanty J, Balkhi MH, Bhat FA, Chishti I (2013) Purification of Schizothorax Immunoglobulin Raised Against Metazoan Parasite: A Preliminary Study. J Fisheries Livest Prod 1:105. doi:10.4172/2332-2608.1000105
Copyright: © 2013 Shah FA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Attempts were made to purify immunoglobulins from serum of Schizothorax, Schizothorax niger, which had been immunized with Pomphorhynchus (Acanthocephalan) antigen. Initially, the protein in the serum was salted out at 50% saturation with ammonium sulphate and was chromatographed successively by gel filtration and ion exchange columns. The Pomphorhynchus kashmirensis agglutination positive fractions from ion exchange column when concentrated and checked in SDS-PAGE, three bands where observed. Since teleosts immunoglobulins have been shown to belong to a single class, the two extra bands found in our study might be the degradation products of immunoglobulin or some unpurified contaminants.