Selected Reaction Monitoring: A Valid Tool for Targeted Quantitation of Protein Biomarker Discovery
Subodh Kumar* and Priyanka Mittal
Central Research Laboratory, Multi-disciplinary Research Unit, University College of Medical Sciences (University of Delhi) and GTB Hospital, Delhi, India
- *Corresponding Author:
- Subodh Kumar
Research Scientist, Multi-Disciplinary Research Unit
UCMS and GTB Hospital
University of Delhi, Delhi-110 095, India
E-mail: [email protected]
Received Date: April 14, 2017 Accepted Date: May 08, 2017 Published Date: May 12, 2017
Citation: Kumar S, Mittal P (2017) Selected Reaction Monitoring: A Valid Tool for Targeted Quantitation of Protein Biomarker Discovery. J Anal Bioanal Tech 8: 362. doi: 10.4172/2155-9872.1000362
Copyright: © 2017 Kumar S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Biomarker discovery is relying on the sensitivity and specificity of the detected biomolecules in clinical samples. Several potential biomarkers against the particular disease cannot be detected because of the unavailability of either specific procedure or sensitive instrument or both, which hamper the diagnosis. In spite of that some of the potential biomarkers are available in trace amount in biological fluids like serum, urine, buccal swab, sputum etc. and need a sensitive method to detect precisely those biomolecules. Now days Selected Reaction Monitoring (SRM) is Mass Spectrometry based approached who can overcome the problems associated with biomarker discovery. India have adequate and variety of clinical samples but some time due to lack of infrastructure and knowledge we are unable to utilise those samples for human welfare. In this review, we are discussing about the experimental setup and procedure of SRM experiment.