Table Sugar as Low Cost Option and its Concentration Effect on Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Genotypes
Received Date: Nov 14, 2016 / Accepted Date: Dec 17, 2016 / Published Date: Dec 22, 2016
Most developing countries are hesitated in maximizing tissue culture technology due to the overhead costs involved. Therefore, this research was initiated to evaluate alternative cheap carbon sources and energy in culture media in order to reduce production input costs of sugarcane in vitro propagation. The experiment was carried out in completely randomized design (CRD) with 2 × 6 factorial treatment arrangements of genotypes and carbon source in combination. The interaction analysis of genotypes and table sugar concentration significantly influenced in vitro sugarcane multiplication. On MS medium with 50 gl-1 table sugar, B4906 gave the highest (13.42 ± 0.29) shoots, whereas Pr1013 produced a maximum of 7.78 ± 0.19 shoots at 60 gl-1 table sugar. However, 40 gl-1 table sugar was optimum to produce usable and separable shoots for further subculture of multiplication. Accordingly results showed that table sugar not only enhanced multiplication but also significantly reduced the production input costs by 94.89% when compared with the analytical grade sucrose.
Keywords: Genotypes; Table sugar; Multiplication; Saccharum officinarum
Citation: Getnet B, Bantte K, Diro M (2016) Table Sugar as Low Cost Option and its Concentration Effect on Shoot Multiplication of Sugarcane (Saccharum officinarum L.) Genotypes. Adv Crop Sci Tech 4:248. Doi: 10.4172/2329-8863.1000248
Copyright: © 2016 Getnet B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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