Abstract

Cellular signaling pathways play a crucial role in regulating cellular responses and maintaining homeostasis. Investigating these pathways is essential for understanding the complex mechanisms underlying cell behavior and disease development. This abstract provides an overview of the investigation of cellular signaling pathways, including the tools, techniques, and recent advancements in this field. Genetic manipulation, pharmacological tools, biochemical analysis, and live-cell imaging are some of the approaches used to dissect signaling pathways. Recent advancements include single-cell analysis, systems biology approaches, and Optogenetics, which have revolutionized our understanding of signaling heterogeneity, network dynamics, and precise manipulation of signaling events. The investigation of cellular signaling pathways holds immense promise for advancing our knowledge of cellular processes and developing therapeutic interventions.