ISSN: 2168-9652

Biochemistry & Physiology: Open Access
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  • Research Article   
  • Biochem Physiol 2019, Vol 8(1): 248
  • DOI: 10.4172/2168-9652.1000248

U-74389G Significantly Reduced TNF-α in a Swine Model of Acute Liver Ischemia-Reperfusion Injury

Periklis Zavridis1*, Georgios Vlachos2, Anthony Lisacek-Kiosoglous3, Andrew Georgiou3, Maria Tsitskari4, Constantinos Hadjileontis5, Ioannis Patrikios3, Apostolos Papalois2,6, George Zografos2 and Konstantinos Toutouzas2
1American Medical Center, Nicosia, Cyprus
2”Ippokrateio” General Hospital of Athens, Department of Surgery, Athens, Greece
3School of Medicine, European University Cyprus, Nicosia, Cyprus
4Apollonion Private Hospital, Nicosia, Cyprus
5Private Histopathologist, Nicosia, Cyprus
6Educational and Research Center ELPEN, Athens, Greece
*Corresponding Author : Periklis Zavridis, American Medical Center, Nicosia, Cyprus, Tel: +357-99603148, Email: periklis@painclinic.com.cy

Received Date: Jan 18, 2019 / Accepted Date: Feb 05, 2019 / Published Date: Feb 13, 2019

Abstract

Introduction: Ischemia-reperfusion injury (IRI) is one of the main causes of increased morbidity in hepatic surgery following portal triad clamping and blood reflow.
Aim: The aim of this study was to determine whether intraoperative administration of the lazaroid U-74389G can reduce inflammatory activity in landrace pigs undergoing hepatectomy with Pringle maneuver.
Methods: Fourteen landrace pigs (30 ± 2 kgr) were randomized into two groups: in group A (n=7) lazaroid U-74389G was administrated immediately after the Pringle maneuver was removed while in group B (control group, n=7) it was not. Blood samples were obtained at four phases: 1) before clamping 2) 30 min after clamping removal 3) two hours after hepatectomy and 4) 24 hours after hepatectomy. Quantitative analyses of the inflammatory markers IL-1β, IL-10, IFN-α, IFN-γ, TNF-α, IL-4, and IL-8 were performed. In order to histopathologically evaluate the number of neutrophils, lymphocytes, macrophages and apoptotic bodies in liver in the two groups, samples which included at least one big vessel were taken at phases 3 and 4.
Results: Histological analysis revealed the presence of inflammation and apoptosis, of the same density, in both groups. Biochemical analysis revealed a statistically significant decrease (P<0.01) of TNF-α at phases 2, 3 and 4 and of IFN-α (p= 0.02) at phase 4 in group A compared to control group.
Conclusion: Pro-apoptotic chemokine TNF-α was significantly reduced for 24 hours after the administration of antioxidant U-74389G in group A. However, the effect of U-74389G did not appear to be obvious in terms of inflammation and apoptosis in histological evaluation.

Keywords: Lazaroid; U-74389G; Liver; Swine; Reperfusion; TNF-α

Citation: Zavridis P, Vlachos G, Lisacek-Kiosoglous A, Georgiou A, Tsitskari M, et al. (2019) U-74389G Significantly Reduced TNF-α in a Swine Model of Acute Liver Ischemia-Reperfusion Injury. Biochem Physiol 8: 248. Doi: 10.4172/2168-9652.1000248

Copyright: © 2019 Zavridis P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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