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A Simple Hollow Fiber Liquid Phase Micro-extraction Combined With HPLC-UV Method For The Extraction, Pre-concentration And Determination Of Olanzapine In The Biological Samples | 10263
ISSN: 2155-9872
Journal of Analytical & Bioanalytical Techniques
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A simple hollow fiber liquid phase micro-extraction combined with HPLC-UV method for the extraction, pre-concentration and determination of olanzapine in the biological samples
4th International Conference and Exhibition on Analytical & Bioanalytical Techniques
Mahnaz Qomi, Elmira Khajouikermani, Kourosh Lozani and Farhad Raofie
Olanzapine (OZP) is a selective monoaminergic antagonist with high binding affinity to serotonin, dopamine and muscarinic
receptors. It has been proposed that this drug?s efficacy in schizophrenia is mediated through a combination of dopamine
and serotonin type 2 antagonism. A new method based on three-phase hollow-fiber liquid phase micro-extraction combined
with high performance liquid chromatography with UV detection (HF-LPME-HPLC-UV) was used for the extraction, pre-
concentration and determination of OZP in urine and plasma samples. Experimental parameters which control extraction
efficiency, such as type of organic solvent, pH of donor and acceptor phase, ionic strength of the sample, stirring rate, extraction
temperature and extraction time were studied. The limit of detection and relative standard deviation (n=5) of the method were
2 ngmL
-1
and 2.1%. Under the optimized conditions, enrichment factors up to 110 were achieved. Olanzapine was successfully
determined in urine and plasma samples without any significant matrix effect.
Biography
Mahnaz Qomi has done Ph.D. in analytical chemistry from Research and Sciences of Islamic Azad University, Tehran, Iran. She is an assistant
professor for 5 years in Pharmaceutical Sciences Branch of Islamic Azad University, Tehran, Iran. She is a researcher manager in the Pharmaceutical
Sciences Research Center at this university with more than 8 published papers in reputed journals.
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