Journal of Analytical & Bioanalytical Techniques
Open Access

Abstract

In the present work, a highly sensitive and selective biosensor for directly electrochemical detection of bisphenol A was developed based on aptamer-functionalized nanoporous gold. Nanoporous gold was prepared by dealloying Ag from Au/Ag alloy leaves in concentrated nitric acid. Nanoporous gold was attached onto glassy carbon electrode and then functionalized with bisphenol A-specific aptamer via the formation of Au-S bond. The morphology of fabricated sensor was studied by SEM and the immobilization of aptamer was demonstrated by XPS. Nanoporous gold exhibited excellent catalytic activity toward the redox reaction of BPA captured by aptamer, which led to ultrasensitivity. Moreover, the aptamer immobilized on nanoporous gold improved the selectivity of the developed biosensor. The experimental parameters in terms of aptamer concentration, reaction time, pH, and temperature were optimized. The calibration curve was constructed and the detection limit was determined to be at subfemtomole. The developed biosensor showed promising potential in real sample analysis.

Biography

Ye Zhu is currently a lecturer in the School of Chemistry and Chemical Engineering at Shandong University, China. She received her BE and MSc from Chongqing University, China. She was awarded her PhD in 2012 from Pusan National University, South Korea. She has published several papers in reputed journals. Her current research interest is mainly focused on the development of electrochemical biosensors for the detection of environmental pollutants and disease biomarker