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Biological mass spectrometry and its applications in proteomics and structural biology. Specifically, he employ multi-dimensional liquid chromatography coupled with electrospray ion trap-Orbitrap mass spectrometry for the analysis of purified proteins, protein complexes, and single/multi-species proteomes.
Major focus of his research is the development and application of proteomics technologies to the understanding of microbial physiology through the analysis of whole proteomes or enriched protein complexes.
Develop new proteome methodologies that will allow for the study of microbes and microbial communities directly from environmental samples, a new subfield termed metaproteomics
Characterization of Clostridium acetobutylicum Protein Interaction Network from Butanol Fermentation
Kumaran Sivagnanam, Vijaya G.S. Raghavan, Manesh Shah, Robert L Hettich, Nathan C Verberkmoes and Mark G Lefsrud
Research Article: J Anal Bioanal Tech 2013, S3:002
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