alexa Luciferase Expression is more Accurate than GFP to Assess Mirnas- Relevant Oncogenesis in vivo Live Imaging Study | OMICS International
1165-158X
Cellular and Molecular Biology
Make the best use of Scientific Research and information from our 700+ peer reviewed, Open Access Journals that operates with the help of 50,000+ Editorial Board Members and esteemed reviewers and 1000+ Scientific associations in Medical, Clinical, Pharmaceutical, Engineering, Technology and Management Fields.
Meet Inspiring Speakers and Experts at our 3000+ Global Conferenceseries Events with over 600+ Conferences, 1200+ Symposiums and 1200+ Workshops on
Medical, Pharma, Engineering, Science, Technology and Business

Luciferase Expression is more Accurate than GFP to Assess Mirnas- Relevant Oncogenesis in vivo Live Imaging Study

Visit for more related articles at Cellular and Molecular Biology

Abstract

Background: In vivo live imaging technology was able to assess dynamically tumor processes and biology throughout the entire experiment period depending on a bioluminescent signal from tumor cells generated from expression of Green Fluorescent Protein (GFP) or the firefly luciferase gene. Recently, there is scarely reported a comparison of Luc- and GFP-transfected tumors to assess the biological mechanism of miRNAs using identical model systems.

Methods: We constructed the SGC-7901 cells which involving stability-enhanced Luc- and GFP-coexpression identified by real time PCR (qPCR) and fluorescence microscope, the constructed cells were injected into the nude mice, then we compared the transfection efficiency and accuracy of GFP and luciferase for miRNAs-relevant oncogenesis after 40 days.

Results: We constructed successfully SGC-7901 cell lines coexpressing the stability-enhanced luciferase and GFP. The miR-145 expression of Luc/LV-miR-145 SGC-7901 cells was higher than Luc/LV-ctrl’s, and luciferase intensity was consistent with tumor volume and negatively related to miR-145 expression in Luc-transfected SGC-7901 cells, but the phenomenon of GFP was opposite. Conclusion: We believed that luciferase offers distinct advantages over GFP as a transfection and gene expression reporter to assist the studies of miRNAs-relevant oncogenesis in vivo live imaging studies.

Select your language of interest to view the total content in your interested language
Post your comment

Share This Article

Relevant Topics

Article Usage

  • Total views: 11502
  • [From(publication date):
    January-2014 - Dec 15, 2017]
  • Breakdown by view type
  • HTML page views : 7760
  • PDF downloads : 3742
 

Post your comment

captcha   Reload  Can't read the image? click here to refresh

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version