ISSN: 2476-2024

Diagnostic Pathology: Open Access
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  • Research Article   
  • Diagnos Pathol Open,
  • DOI: 10.4172/2476-2024.1000212

A Comparative Study of the Fixative Properties of Honey and Formalin

Owusu Afriyie Bright1,3,5*, Osei Sarpong Albert1, Addai Kusi Michael2, Quartey Perez1, Owusu Ohui Dorcas4, Antwi Melody Ama5, Ocran Tiwaa Josephine5, Mensah Arhin Elisha5, Brenya Antwi Godfred5, Adu Acheampomaa Josephine5, Adjetey Caleb Sowah5 and Agyemang Mary-Kezia Pokuaa5
1Department of Medical Laboratory Technology, Garden City University College, Kumasi, Ghana
2Department of Medical Laboratory, Hope Xchange Medical Centre, Kumasi, Ghana
3Department of Medical Laboratory Science, University of Energy and Natural Resources, Sunyani, Ghana
4Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
5Department of Pathology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
*Corresponding Author : Dr. Owusu Afriyie Bright, Department of Medical Laboratory Technology, Garden City University College, Kumasi, Ghana

Received Date: Dec 23, 2022 / Accepted Date: Jan 16, 2023 / Published Date: Jan 23, 2023

Abstract

Before a routine histological or cytological examination, tissues are fixed, maintained, and guarded against putrefaction and autolysis. The fixative is most frequently used to fix tissues in formalin. Because of its toxicity using formalin poses health risks, according to the Occupational Safety and Health Administration (OSHA) we want to use a natural alternative such as honey in a formalin-free laboratory for the preservation of clinical specimens. Honey is a naturally occurring substance with antibacterial, acidic and dehydrating qualities that make it a fixative. This study compares the effectiveness of formalin and honey as fixative agents. A rat liver tissue sample, formalin, honey, alcohol, xylene, cassette, mold, paraffin wax, rotary microtome, floating bath, slides, rack, and hematoxylin and eosin for staining are all required for this study. A liver tissue biopsy will be fixed with formalin and honey respectively for 24 hours while being grossed and recorded every four hours. It will go through dehydration, clearing, grossing, paraffin wax, hematoxylin, eosin staining and microscopic examination. The anticipated outcomes will demonstrate statistically significant changes in nuclear specificity and cytoplasmic staining between honey and formalin samples. A a substitute for formalin and as a tissue fixative honey is readily available has no known toxicity, and can be employed. However research should be done to identify ways to get rid of the drawbacks, including homogenization, seen with connective tissue.

Keywords: Formalin; Fixation; Honey; Tissue fixative; Tissue processing; H and E staining

Citation: Bright OA, Albert OS, Michael AK, Perez Q, Dorcas OO, et al. (2023) A Comparative Study of the Fixative Properties of Honey and Formalin. Diagnos Pathol Open 8:212. Doi: 10.4172/2476-2024.1000212

Copyright: © 2023 Bright OA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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