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Hindi Research Article
A Comparison Between Two Assays for the Redox Status in Plasma
Eugene Jansen1*, Piet Beekhof1, Nicole Schupp2, Moritz Kreutzmann2 and Bettina Kraus3
1Centre for Health Protection, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
2Institute of Toxicology, University of Düsseldorf, Germany
3Department of Internal Medicine I, University Hospital Würzburg, Germany
- *Corresponding Author:
- Eugene Jansen
Centre for Health Protection
National Institute for Public Health and the Environment
Bilthoven, The Netherlands
Tel: +31-30-2742940
E-mail: eugene.jansen@rivm.nl
Received date: December 20, 2016; Accepted date: January 04, 2017; Published date: January 09, 2017
Citation: Jansen E, Beekhof P, Schupp N, Kreutzmann M, Kraus B (2017) A Comparison Between Two Assays for the Redox Status in Plasma. J Anal Bioanal Tech 8:342. doi: 10.4172/2155-9872.1000342
Copyright: © 2017 Jansen E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
In the present study, two commercial assays for the redox status are compared and evaluated, the SH groups in proteins (SHp) and the total thiol levels (TTL). Both assays are colorimetric endpoint reactions and have been adapted for use on an automatic clinical analyzer. Both assays performed well by giving comparable results in plasma samples. A good correlation with a correlation coefficient (R2) of 0.889 was found between the two assays. However, for some pathological specimens, especially highly lipemic samples, the SHp assay performed better than the TTL assay. When plasma samples with a high lipemic index were omitted, the correlation between the two assays was even better with an R2 of 0.942.
In conclusion, both assays for the redox status performed well, but the SHp assay can also be used in high lipemic samples.
