A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic StudyShashidhar Reddy D1*, Prakash Rao2 and Devaraj VC1
- *Corresponding Author:
- Dr. Shashidhar Reddy.D
Dept. of Pharmacology
Bioneeds Laboratory Animals & Preclinical Services
Bangalore: 562111, Karnataka, India
Tel: +91 8374093233, +91 8453336609
E-mail: [email protected], [email protected]
Received date: September 07, 2011; Accepted date: October 07, 2011; Published date: October 25, 2011
Citation: Reddy SD, Rao P, Devaraj VC (2011) A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic Study. J Anal Bioanal Tech 2:125. doi: 10.4172/2155-9872.1000125
Copyright: © 2011 Reddy SD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A highly senstive and rapid LC-MS/MS method has been developed and validated for the estimation of desvenlafaxine in rabbit plasma. The chromatographic separation was performed with 0.2% formic acid: methanol at flow rate of 0.4 mL/min on Symmetry Shield RP18 column with a total run time of 3.0 min. TheMS/MS ion transitions monitored were 263.90? 58.10 for desvenlafaxine and 281.30--> 86.10 for IS (metaprolol). Method validation and pre-clinical sample analysis were performed as per FDA guide lines and the results met the acceptance crieteria. The lower limit of quantification achieved was 0.5 ng/mL and the linearity was observed from 0.5 to 1500 ng/mL. This novel method has been applied to pharmacokinetic study of desvenlafaxine in rabbits.