Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic Study

¹Department of Pharmacology, Bioneeds Preclinical Services, Bangalore, India

²Department of Pharmaceutics, Karnataka College of Pharmacy, Bangalore, India

*Corresponding Author:

Dr. Shashidhar Reddy.D
Research Scholar
Dept. of Pharmacology
Bioneeds Laboratory Animals & Preclinical Services
Bangalore: 562111, Karnataka, India
Tel: +91 8374093233, +91 8453336609
Fax: 914067204199
E-mail: dasdev@in.com, devcology@yahoo.com

Received date: September 07, 2011; Accepted date: October 07, 2011; Published date: October 25, 2011

Citation: Reddy SD, Rao P, Devaraj VC (2011) A Highly Sensitive LC-Ms/Ms Method for Determination of Desvenlafaxine in Rabbit Plasma and its Application to Rabbit Pharmacokinetic Study. J Anal Bioanal Tech 2:125. doi: 10.4172/2155-9872.1000125

Copyright: © 2011 Reddy SD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

A highly senstive and rapid LC-MS/MS method has been developed and validated for the estimation of desvenlafaxine in rabbit plasma. The chromatographic separation was performed with 0.2% formic acid: methanol at flow rate of 0.4 mL/min on Symmetry Shield RP18 column with a total run time of 3.0 min. TheMS/MS ion transitions monitored were 263.90? 58.10 for desvenlafaxine and 281.30--> 86.10 for IS (metaprolol). Method validation and pre-clinical sample analysis were performed as per FDA guide lines and the results met the acceptance crieteria. The lower limit of quantification achieved was 0.5 ng/mL and the linearity was observed from 0.5 to 1500 ng/mL. This novel method has been applied to pharmacokinetic study of desvenlafaxine in rabbits.

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