alexa A HPLC Method for Determination of Ursolic Acid and Betulinic Acids from their Methanolic Extracts of Vitex Negundo Linn | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Research Article

A HPLC Method for Determination of Ursolic Acid and Betulinic Acids from their Methanolic Extracts of Vitex Negundo Linn

S. V. Taralkar1*and S. Chattopadhyay2

1Chemical Engineering Department, MAEER’s Maharashtra Academy of Engineering, Alandi (D), Pune-412105, Maharashtra, India

2Polymer Engineering Department, Indian Institute of Technology, Roorkee-247667, Uttarakhand, India

*Corresponding Author:
S. V. Taralkar
Chemical Engineering Department
MAEER’s Maharashtra Academy of Engineering
Alandi (D), Pune-412105
Maharashtra, India
Tel: +912030253621, +919011332500
Fax No:
E-mail: [email protected]

Received date: April 23, 2012; Accepted date: June 01, 2012; Published date: June 07, 2012

Citation: Taralkar SV, Chattopadhyay S(2012) A HPLC Method for Determination of Ursolic Acid and Betulinic Acids from their Methanolic Extracts of Vitex Negundo Linn. J Anal Bioanal Tech 3:134. doi: 10.4172/2155-9872.1000134

Copyright: © 2012 Taralkar SV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Ursolic and betulinic acids are very useful nutraceuticals available in herbs. Their quantitative estimation from the solvent extracts of the herbs is a great challenge. So far all the chromatographic methods available for identification and quantification of these acids are distinctly different, meaning thereby, each acid would require a separate method. Reverse phase (RP-HPLC) method is developed for determination of ursolic acid and betulinic acid from their methanol extract of Vitex negundo Linn leaves. Analysis was carried out using Waters’ symmetry C-18 column with acetonitrile: methanol (80:20) as isocratic elution mode with UV detection (λ=210 nm). The method is pretty linear for ursolic acid in the range of 0.01-0.1 mg/ml (R2 = 0.9961) and for betulinic acid in the range of 0.003-0.018 mg/ml (R2 = 0.999). The peaks of ursolic acid and betulinic acid were confirmed by LC-MS. The method was validated by mixing these acids standards in methanol and found that it is accurate, sensitive and has a good reproducibility.


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