A Novel Method for the Determination of Total Hydrocarbon in the Hydrocarbon Mixture-Contaminated Soil | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

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Special Issue Article

A Novel Method for the Determination of Total Hydrocarbon in the Hydrocarbon Mixture-Contaminated Soil


A simple, reliable and rapid analysis method, for the determination of total hydrocarbon content in a hydrocarbon mixture-contaminated soil that derived from a drum washing factory, was described and validated for the remediation of the contaminated site. This method was based on an assumption that the possibly extracted total hydrocarbon by organic solvent in the hydrocarbon mixture-contaminated soil had a ultraviolet absorption peak and could be used as hydrocarbon mixture standard sample (MSS), by which total hydrocarbon content in the contaminated soil could be measured using ultraviolet spectrophotometry instead of traditional analysis methods (GC, HPLC, GC-MS and HPLC-MS). The results shown that after the available MSS was dissolved in dichloromethane, it stably exhibited ultraviolet absorption peak at 230 nm and the calibration curve for absorbance versus concentrations indicated a high correlation (r=0.9999, p<0.001). The recoveries of this method were in the range of 81.43-104.49% with total hydrocarbon content ranging from 150 to 9000 mg kg-1 when extracted with pure dichloromethane; and the soil organic matter (SOM) interference with this method was negligible due to the percentage of transported concentration of SOM in total hydrocarbon content of contaminated soil was less than 1%. In general, the proposed method had the advantages of convenience, simplicity and good repeatability, and could be used to rapidly determine the total hydrocarbon content in hydrocarbon mixture-contaminated soils in this study. However, heavy metals exhibited interference in this method and it was necessary to analyze the existence of heavy metals prior to the adoption of this method.