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Hindi Research Article
A Portable Microcontrolled Pumping Flow System Developed to In Loco Determination of Diuron at Nanomolar Levels Employing a Disposable Biosensor
Bruno C Janegitz*, Fernando Campanhã Vicentini, Vagner B dos Santos, Thiago B Guerreiro and Orlando Fatibello-Filho
Department of Chemistry, Center of Exact Sciences and Technology, Universidade Federal de São Carlos, São Carlos - SP, Brazil
- *Corresponding Author:
- Bruno C Janegitz
Department of Chemistry
Center of Exact Sciences and Technology
Universidade Federal de São Carlos
P. O. Box 676, 13560-970, São Carlos - SP, Brazil
Tel: +55-163-351-8098
Fax: +55-163-351-8350
E-mail: brunocj@ymail.com
Received date: February 24, 2015; Accepted date: May 19, 2015; Published date: May 26, 2015
Citation: Janegitz BC, Vicentini FC, Santos VB, Guerreiro TB, Fatibello-Filho O (2015) A Portable Microcontrolled Pumping Flow System Developed to In Loco Determination of Diuron at Nanomolar Levels Employing a Disposable Biosensor. J Anal Bioanal Tech 6: 247. doi: 10.4172/2155-9872.1000247
Copyright: © 2015 Janegitz BC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Microcontrolled systems coupled biosensors have attracted attention. Here, we describe an automated and portable microcontrolled pumping flow system (μC-PFS) developed to biosensing application. The flow system is fully automatic, portable, it works in battery module and it dispenses the use of microcomputer and the manual operations. This device presents mainly a microcontroller as central processing unit (CPU), an aquarium air pump and three-way solenoid valves to propulsion and the volume control of aliquots inserted in the flow system. Once available its performance, this device was used to pump samples and solution towards a compacted and miniaturized electrochemical flow cell (EFC) employing a multicommutated flow procedure. In the EFC, it was used a biosensor based on acetylcholinesterase immobilized on the gold nanoparticles modified-screen printed carbon electrode, which was used in order to perform the determination of diuron by enzymatic inhibition employing a chronoamperometry. Under the best experimental conditions, the calibration curve for diuron determination was linear in the concentration range from 80 to 1400 nmol/L with a detection limit of 50 nmol/L. Moreover, the proposed system was fully automated, showed precise, with low consumption of sample and waste generation with an analytical throughput of 13/h.
