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A Ringdown Breath Acetone Analyzer: Performance and Validation Using Gas Chromatography-Mass Spectrometry | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

A Ringdown Breath Acetone Analyzer: Performance and Validation Using Gas Chromatography-Mass Spectrometry

Gong ZY1, Sun MX1, Jian CY1, Wang ZN1, Kang ML1, Li YX1 and Wang C1,2*

1Laser Medicine Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin, China

2Department of Physics and Astronomy, Mississippi State University, USA

*Corresponding Author:
Chuji Wang
Professor, Department of Physics and Astronomy
Mississippi State University
Starkville, MS 39759
US Army Research Laboratory
Adelphi, MD 20783, USA
Tel: 662-325-9455
E-mail: [email protected]

Received Date: March 17, 2014; Accepted Date: April 25, 2014; Published Date: April 28, 2014

Citation: Gong ZY, Sun MX, Jian CY, Wang ZN, Kang ML, et al. (2014) A Ringdown Breath Acetone Analyzer: Performance and Validation Using Gas Chromatography- Mass Spectrometry. J Anal Bioanal Tech S7:013. doi: 10.4172/2155-9872.S7-013

Copyright: © 2014 Gong ZY, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acetone in exhaled breath is a potential biomarker of diabetes mellitus (DM) and elevated breath acetone concentrations have been observed in DM patients. One of the near real-time and online breath acetone analysis techniques is cavity ringdown spectroscopy (CRDS) that has been demonstrated for breath acetone measurements in human subjects including diabetic patients in a clinic. In this work, we have constructed a ringdown breath acetone analyzer for the purpose of instrument validation. Its detection capabilities, such as limit of detection, baseline stability, detection sensitivity, and reproducibility, were investigated. For the first time, a ringdown acetone breath analyzer has been validated using gas chromatography-mass spectrometry (GC-MS), which is typically referred to as the golden standard method for trace gas analysis. The GC-MS validation challenged the analyzer’s response to various breath acetone concentrations and its quantitative measurement accuracy. Subsequently, 25 subjects including 19 healthy and 6 diabetic people were tested using the validated ringdown breath acetone analyzer. Comparison of the testing results shows that this ringdown breath acetone analyzer can be used for reliable real-time, online breath acetone analysis in a clinic.


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