A Simple and Rapid LC-MS/MS Method for the Determination of BMCL26 a Novel Anti-Parasitic Agent in Rat Plasma
Ramakrishna R Voggu, Xiang Zhou, Bin Su and Baochuan Guo*
Department of Chemistry, Cleveland State University, USA
- *Corresponding Author:
- Baochuan Guo
Department of Chemistry
College of Science and Health Professions
Cleveland State University, 2121 Euclid Avenue,
Cleveland, Ohio, 44115, USA
E-mail: [email protected]
Received date: July 24, 2015; Accepted date: August 08, 2015; Published date: August 15, 2015
Citation: Voggu RR, Zhou X, Su B, Guo B (2015) A Simple and Rapid LC-MS/ MS Method for the Determination of BMCL26 a Novel Anti-Parasitic Agent in Rat Plasma. J Anal Bioanal Tech 6:265 doi:10.4172/2155-9872.1000266
Copyright: © 2015 Voggu RR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
BMCL26 is a potential drug derived from nimesulide, which has exhibited the substantial anti-parasitic activity in various cell lines. To conduct various pharmacological and toxicological properties of this drug, we developed and validated a rapid LC-MS/MS method for its quantification in accordance with the FDA guidelines. Protein precipitation with 0.1% formic acid in acetonitrile was used to extract the analytes along with the internal standard (JCC76) from rat plasma. It was found that the calibration curve of the method had an excellent linearity (r2 ≥ 0.9993) for the analyte concentration ranging from 0.5 to 100 ng/mL with acceptable inter- and intra-assay, precision, accuracy and stability. The matrix effect and extraction recovery were in the range of 101.30-110.10% and 90.16- 105.00%, respectively. This LC-MS/MS method is simple and rapid and can be used in the future pharmaceutical studies of BMCL26.