Advanced LC-MS/MS Techniques Dissecting Diverse Isomers of Plant Sphingolipid SpeciesToshiki Ishikawa1, Daiki Yanagawa2, Maki Kawai-Yamada1 and Hiroyuki Imai2*
1Graduate School of Science and Engineering, Saitama University, Japan
2Department of Biology, Graduate School of Natural Science, Konan University, Japan
- *Corresponding Author:
- Hiroyuki Imai
Department of Biology, Graduate School of Natural Science
Konan University, 8-9-1 Okamoto
Higashinada-ku, Kobe 658-8501, Japan
E-mail: [email protected]
Received date: December 26, 2013; Accepted date: January 25, 2014; Published date: January 28, 2014
Citation: Ishikawa T, Yanagawa D, Kawai-Yamada M, Imai H (2014) Advanced LC-MS/MS Techniques Dissecting Diverse Isomers of Plant Sphingolipid Species. J Anal Bioanal Tech S5:007. doi: 10.4172/2155-9872.S5-007
Copyright: © 2014 Ishikawa T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sphingolipid is a major lipid class ubiquitously present in eukaryotes and several species of prokaryotes. Recent genetic and sphingolipidomic researches have been revealing complex structures of plant sphingolipids and their functions in various aspects of plant biological events as major membrane components as well as signaling molecules. Particularly, cis/transisomeric double bond at Δ8 position of long-chain bases (LCBs) is of great interest due to their potential impacts on stress tolerance in plants and yeast. In addition, free LCBs and their metabolism are also important for intracellular signaling pathways during various environmental stresses. In this review, we introduce two current improvements of sphingolipidomic analyses based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), focusing on high-throughput quantitative analysis of complex structural isomers of plant sphingolipids.