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ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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Research Article

Antibiotic Susceptibility and Heavy Metal Tolerance Pattern of Serratia Marcescens Isolated From Soil and Water

Natasha Nageswaran1, P.W. Ramteke2, O.P. Verma*3 and Avantika Pande3
1Department of Microbiology and Fermentation Technology, Jacob School of Biotechnology & Bioengineering, SHIATS Allahabad-211007, Uttar Pradesh, India
2Department of Biological Sciences, Jacob School of Biotechnology & Bioengineering, SHIATS Allahabad-211007, Uttar Pradesh, India
3Department of Molecular & Cellular Engineering, Jacob School of Biotechnology & Bioengineering, SHIATS Allahabad-211007, Uttar Pradesh, India
Corresponding Author : O.P. Verma
Department of Molecular & Cellular Engineering
Jacob School of Biotechnology & Bioengineering
SHIATS Allahabad-211007
Uttar Pradesh, India
E-mail: [email protected]
Received May 03, 2012; Accepted June 20, 2012; Published June 22, 2012
Citation: Nageswaran N, Ramteke PW, Verma OP, Pandey A (2012) Antibiotic Susceptibility and Heavy Metal Tolerance Pattern of Serratia marcescens Isolated From Soil and Water. J Bioremed Biodeg 3:158. doi: 10.4172/2155-6199.1000158
Copyright: © 2012 Nageswaran N, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The antibiotic and metal tolerance patterns of Serratia marcescens strains isolated from soil and water around the Sangam region of Allahabad were obtained. Using the standard minimum inhibitory concentration (MIC) for each antibiotic respectively, the Kirby-Bauer disc-diffusion method was used to obtain antibiotic resistance patterns of the Serratia strains and the MIC of the metals - chromium, cadmium, cobalt, copper, lead and nickel for each of the strains were also obtained. Plasmid curing was carried out for specific antibiotic and metal resistances to ascertain plasmid-borne transfer of resistance genes. Results obtained showed that Multi-drug resistant (MDR) strains of Serratia were resistant to certain metals as well suggesting specific metal-antibiotic resistant gene patterns in the different strains.

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