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Bioanalytical Method Development and Validation for Latanoprost Quantification in Pharmaceutical Opthalmic Microemulsion Formulation by RP-HPLC | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Research Article

Bioanalytical Method Development and Validation for Latanoprost Quantification in Pharmaceutical Opthalmic Microemulsion Formulation by RP-HPLC

Pratikeshkumar Patel1, Priti Patel2 and Prabhanjan Giram1*

1Polymer Science and Engineering, CSIR- NCL, Pune, Maharashtra, India

2Sayajirao University of Baroda, Gujarat, India

*Corresponding Author:
Prabhanjan S Giram
CSIR- National Chemical Laboratory (NCL)
Dr. Homi Bhabha Road, Pune-411 008, India
Tel: +9120-25902000/25893400
Fax: +9120-25902660
E-mail: [email protected]

Received date: August 25, 2015; Accepted date: October 12, 2015; Published date: October 19, 2015

Citation: Patel P, Patel P, Giram P (2015) Bioanalytical Method Development and Validation for Latanoprost Quantification in Pharmaceutical Opthalmic Microemulsion Formulation by RP-HPLC. J Anal Bioanal Tech 6:284. doi:10.4172/2155-9872.1000284

Copyright: © 2015 Patel P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A novel HPLC method has been developed and validated for the determination of Latanoprost in pharmaceutical ophthalmic formulation for in-vivo and ex-vivo animal studies. The method used a Supelco analytical C18 (250 mm × 4.6 mm i.d., 5 μm particle size) column, under the following chromatography conditions: Isocratic elution, using a mobile phase composed by consisting of acetonitrile as an organic modifier and water of 60:40 (v/v). The mobile phase was delivered at a flow rate of 1 ml/min and the detection was made by Ultraviolet (UV) detector at 210 nm. This method was fully validated and applied to available pharmaceutical product. The limit of detection (LOD) and limit of quantitation (LOQ) for Latanoprost were 0.07 μg/ml and 0.019 μg/ml respectively. The purpose of this method analysis of marketed and developed ophthalmic formulations of Latanoprost. This method can be used for quality control assay of Latanoprost in materials as well as in pharmaceutical formulations.

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