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Cardiovascular Drug Medication Adherence Assessed by Dried Blood Spot Analysis | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Cardiovascular Drug Medication Adherence Assessed by Dried Blood Spot Analysis

Sangeeta Tanna and Graham Lawson*

Leicester School of Pharmacy, Faculty of Health and Life Sciences, De Montfort University, UK

*Corresponding Author:
Graham Lawson
Leicester School of Pharmacy, Faculty of Health and Life Sciences
De Montfort University, Leicester, LE1 9BH, UK
Tel: 44-116-257-7129
E-mail: [email protected]

Received date: December 05, 2013; Accepted date: January 27, 2014; Published date: January 29, 2014

Citation: Tanna S, Lawson G (2014) Cardiovascular Drug Medication Adherence Assessed by Dried Blood Spot Analysis. J Anal Bioanal Tech S12: 006. doi: 10.4172/2155-9872.S12-006

Copyright: © 2014 Tanna S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The use of dried blood spot (DBS) collection cards was investigated for the detection of therapeutic drugs used in cardiovascular therapy for assessing medication adherence. A liquid chromatography-high resolution mass spectrometry (LC-HRMS) method was developed for the determination of, bisoprolol, ramipril, and simvastatin. Whole blood spiked with target analytes was used to produce 30 μl blood spots on specimen collection cards for calibration purposes. An 8 mm disc was cut from the dried blood spot and extracted using methanol: water (70:30, v/v). The High Resolution MS detection, at the precise mass of the target drugs, was carried out in electrospray positive ion mode. The LC-HRMS method successfully identified control volunteers who were known to be either adherent or non-adherent. There were no false positives from volunteers taking other cardiovascular drugs or from volunteers receiving no medication. Potentially therefore the system would identify non–adherent patients based on the absence of the target analyte in the DBS sample provided.

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Citations : 3732

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