Abstract

Chassis organisms serve as foundational platforms in synthetic biology, enabling the design and engineering of biological systems for diverse applications. These organisms, typically well-characterized microbes such as Escherichia e coli and Saccharomyces cerevisiae, provide a stable and predictable environment for genetic modifications, facilitating the development of biosynthetic pathways, metabolic engineering, and biomanufacturing. The selection of an optimal chassis depends on factors such as genetic tractability, growth conditions, and biosafety. Advances in genome editing and synthetic biology tools have expanded the repertoire of chassis organisms, including minimal cells and non-model microbes, enhancing their utility in medicine, biofuel production, and environmental biotechnology. This article explores the role of chassis organisms in synthetic biology, highlighting their advantages, current developments, and future potential