alexa Cloning, Sequencing and Expression of Novel Trichloroet
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication

Piyali Mukherjee and Pranab Roy*
Department of Biotechnology, University of Burdwan, Golapbag more, Burdwan-713104, West Bengal, India
Corresponding Author : Pranab Roy
Department of Biotechnology
University of Burdwan, Golapbag more
Burdwan-713104. West Bengal, India
Tel: +9933037099
E-mail: [email protected]
Received: November 24, 2012; Accepted: January 15, 2013; Published: January 17, 2013
Citation:Mukherjee P, Roy P (2013) Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication. J Bioremed Biodeg 4:177. doi:10.4172/2155-6199.1000177
Copyright: © 2013 Mukherjee P, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google
 

Abstract

A bacterium capable of growth on trichloroethylene as the sole carbon source was identified as Stenotrophomonas maltophilia PM102 by 16S rDNA sequencing (GenBank acc. No. JQ797560). Genomic DNA of this bacterium was amplified using primers specific for the original todC1 gene of Pseudomonas putida F1. Two PCR products were obtained: 300 bp and 350 bp respectively, designated as tce300 and tce350 genes. These novel genes were separately cloned into p-GEMT Easy vector: PR300 and PR350 and sequenced. The sequences have been deposited at NCBI GenBank under accession nos. JX910450 and JX910451.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords