alexa Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

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Research Article

Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication

Piyali Mukherjee and Pranab Roy*
Department of Biotechnology, University of Burdwan, Golapbag more, Burdwan-713104, West Bengal, India
Corresponding Author : Pranab Roy
Department of Biotechnology
University of Burdwan, Golapbag more
Burdwan-713104. West Bengal, India
Tel: +9933037099
E-mail: [email protected]
Received: November 24, 2012; Accepted: January 15, 2013; Published: January 17, 2013
Citation:Mukherjee P, Roy P (2013) Cloning, Sequencing and Expression of Novel Trichloroethylene Degradation Genes from Stenotrophomonas maltophilia PM102: A Case of Gene Duplication. J Bioremed Biodeg 4:177. doi:10.4172/2155-6199.1000177
Copyright: © 2013 Mukherjee P, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

A bacterium capable of growth on trichloroethylene as the sole carbon source was identified as Stenotrophomonas maltophilia PM102 by 16S rDNA sequencing (GenBank acc. No. JQ797560). Genomic DNA of this bacterium was amplified using primers specific for the original todC1 gene of Pseudomonas putida F1. Two PCR products were obtained: 300 bp and 350 bp respectively, designated as tce300 and tce350 genes. These novel genes were separately cloned into p-GEMT Easy vector: PR300 and PR350 and sequenced. The sequences have been deposited at NCBI GenBank under accession nos. JX910450 and JX910451.

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