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Comparative Phytochemical Profiling in Swertia Species Collected from Different Geographical Region of India Using High Performance Liquid Chromatography Coupled with Photodiode Array Detector and Mass Spectrometry (HPLC-PDA-MS) Followed by Antioxidant Potential| Abstract

Journal of Phytochemistry & Biochemistry
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  • Research Article   
  • J Phytochemistry Biochem,

Comparative Phytochemical Profiling in Swertia Species Collected from Different Geographical Region of India Using High Performance Liquid Chromatography Coupled with Photodiode Array Detector and Mass Spectrometry (HPLC-PDA-MS) Followed by Antioxidant Potential

Amit Srivastava1, Nishi Srivastava1, Surabhi Tiwari1, Sharad Srivastava1, Bhaskar Dutt2 and AKS Rawat1*
1Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, , Lucknow-226001, India
2Herbarium Section, CSIR-National Botanical Research Institute, , Lucknow-226001, India
*Corresponding Author : AKS Rawat, Division of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow-226001, India, Tel: + 91-522-2297802, Email: [email protected]

Received Date: Jan 12, 2018 / Accepted Date: Jan 29, 2018 / Published Date: Feb 05, 2018

Abstract

The aim of present study is comparative evaluation of iridoid glucosides and flavonoids phytochemicals in different species of Swertia collected from different geographical region in India using HPLC-PDA-MS analytical method and their antioxidant potential. Total seven species of Swertia viz. S. nervosa (SW-1), S. bimaculata (SW- 2) and S. chirata (SW-3), S. ciliata (SW-4), S. paniculata (SW-5), S. cordata (SW-6), and S. alata (SW-7) and six phytochemicals/biological active compounds viz. Swertiamarin, Sweroside, Swertisin, Amarogentin, Luteolin and Genistein have been selected for study. All compounds were separated on C-18 column using mobile phase A (0.5% acetic acid in water) and phase B (100% acetonitrile) in a gradient mode. Total six peaks from six compounds of seven species were unequivocally detected by comparing their UV spectra, retention time, TIC data, and in MS spectra under positive electrospray ionization mode (ESI-MS), fragmentation pattern of all targeted compounds with those of standard compounds. All targeted species were differentiated by variation in relative concentrations of the six compounds. Antioxidant potentials of all selected compounds were also evaluated. Higher total polyphenolic contents is reported in S. chirata and simultaneously validated through antioxidant activity assay such as β-carotene oxidation inhibition, DPPH radicals scavenging activity, and reducing potential in comparison to other species. Our developed and validated analytical methods HPLC-PDA-MS not only facilitate qualitative and quantitative study of targeted compounds in all species of Swertia but have important application in systematic evaluation of distribution of studied iridoids and flavonoid compounds. The developed method can be well employed in the detection and quantitative evaluation of targeted compounds in the bulk samples and different species of Swertia at industrial level.

Keywords: HPLC-PDA-MS; Iridoid compounds; Flavonoid compounds; Swertia species; Antioxidant potential

Citation: Srivastava A, Srivastava N, Tiwari S, Srivastava S, Dutt B, et al. (2018) Comparative Phytochemical Profiling in Swertia Species Collected from Different Geographical Region of India Using High Performance Liquid Chromatography Coupled with Photodiode Array Detector and Mass Spectrometry (HPLC-PDA-MS) Followed by Antioxidant Potential. J Phytochemistry Biochem 2: 105.

Copyright: © 2018 Srivastava A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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