Research Article
Comparative Study of Tissue Distribution of Chlorin e6 Complexes with Amphiphilic Polymers in Mice with Cervical Carcinoma
Marina V Shirmanova1,2*, Alena I Gavrina1,2, Nadeshda A Aksenova3, Nikolay N Glagolev3, Anna B Solovieva3, Boris E Shakhov1 and Elena V Zagaynova1,21Nizhny Novgorod State Medical Academy, Minin and Pozharsky, Russia
2Lobachevsky State University of Nizhny Novgorod, Russia
3Semenov Institute of Chemical Physics RAS, Russia
- *Corresponding Author:
- Marina V Shirmanova, PhD
Head of Laboratory
Nizhny Novgorod State
Medical Academy, Sq. Minin and Pozharsky
10/1, Nizhny Novgorod
603005, Russia
Tel: +7 831 465 4113
Fax: +7 831 465 4223
E-mail:shirmanovam@mail.ru
Received date: April 25, 2014; Accepted date: June 12, 2014; Published date: June 17, 2014
Citation: Shirmanova MV, Gavrina AI, Aksenova NA, Glagolev NN, Solovieva AB,et al. (2014) Comparative Study of Tissue Distribution of Chlorin e6 Complexes with Amphiphilic Polymers in Mice with Cervical Carcinoma. J Anal Bioanal Tech S1:008. doi: 10.4172/2155-9872.S1-008
Copyright: © 2014 Shirmanova MV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Many photosensitizers, including chlorins, are highly hydrophobic, which makes intravenous administration problematic and affects their delivery to the tumor and uptake in the cells. Moreover, self-aggregation of the photosensitizer in aqueous solution reduces fluorescence quantum yield, triplet state, and singlet oxygen generation, and consequently diminishes photosensitizing activity. To address these issues, it was proposed to use biocompatible water-soluble polymers. However, animal studies of the photosensitizer-polymer systems are still very limited. In this work, polyvinylpyrrolidone (PVP), polyvinyl alcohol (PVA), and pluronic F108 were used for dissolution of chlorin e6 (Ce6). Dynamics of accumulation of the formulations in a mouse cervical carcinoma and clearance from normal tissue, drug plasma concentrations and tissue distribution after intravenous injection were investigated. Ce6 alone and clinically used photosensitizer Photoditazine served as a control. The results showed that none of the polymers significantly changed fluorescence kinetics in the tumor. Concentration of the Ce6 formulated with polymers in the tumor tissue was comparable with Photoditazine, but uptake in the skin was less. At the same time, tumor-toskin ratios of the Ce6-polymer complexes were similar to free Ce6. We concluded that the use of the polymeric formulation is reasonable for fluorescence diagnosis and PDT of cancer.
