alexa Comparison of Various Detection Systems Coupled to High Performance Liquid Chromatography for Determination of Tocopherols in Meat. The Influence and Comparison of the Most Popular Sample Preparation Method
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Comparison of Various Detection Systems Coupled to High Performance Liquid Chromatography for Determination of Tocopherols in Meat. The Influence and Comparison of the Most Popular Sample Preparation Method

Szterk A1 *, Roszko M2 , Najman K1 , Kruk M1 , Mroczek E1 , Zarodkiewicz M1 , Rogalski M1 and Waszkiewicz-Robak B1

1Department of Functional Food, Organic Food and Commodities Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences, Poland

2Department of Food Analysis, Institute of Agricultural and Food Biotechnology, Poland

*Corresponding Author:
Arkadiusz Szterk
Department of Functional Food
Organic Food and Commodities Faculty of Human Nutrition and Consumer Sciences
Warsaw University of Life Sciences
159 c Nowoursynowska, 02-776 Warsaw, Poland
Tel: +48 22 59 37057
Fax: +48 22 59 37040
E-mail:[email protected]

Received date: December 04, 2013; Accepted date: December 28, 2013; Published date: December 30, 2013

Citation: Szterk A, Roszko M, Najman K, Kruk M, Mroczek E, et al. (2013) Comparison of Various Detection Systems Coupled to High Performance Liquid Chromatography for Determination of Tocopherols in Meat. The Influence and Comparison of the Most Popular Sample Preparation Method. J Anal Bioanal Tech S2: 005. doi: 10.4172/2155-9872.S2-005

Copyright: © 2013 Szterk A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The presented data are of practical importance in terms of vitamin E determination in meat and other foodstuffs. Selection of a proper separation and detection method as well as sample preparation protocol might affect the reliability of results. Application of a sensitive method and such sample preparation procedures that do not cause significant degradation of the analyte ensures the reliability of the results. The conducted research has proved that the fluorescence detector (FLD) is the most sensitive of the detection systems tested and provides the LOD values in 3.8-38.5 ng ml-1 range. Ion trap was proved to be less a sensitive detector against vitamin E when compared to FLD. When atmospheric pressure chemical ionisation (APCI) was utilised the LOD values observed for γ- and α- tocopherole were 0.2 and 0.5 μg ml-1, respectively. However when atmospheric pressure photo/chemical ionisation (APPI/APCI) was used a significant increase in the ionization efficiency of δ-tocopherol was observed, which caused increases in the instrument sensitivity (LOD=1.0 μg ml-1). Sample preparation protocol based on extraction of the biological sample with ethanol and hexane with simultaneous change in partitioning coefficient, results in significantly higher recovery rates when compared to methods based on sample saponification and extraction with other solvents. Vitamin E contents in the tested pork samples were found to be in the 0.0-6.0 mg kg-1 range, depending on the determination method utilised.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords