alexa Degradation and Colonization of Cellulose by Diazotrophic Strains of Paenibacillus polymyxa Isolated from Soil | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Degradation and Colonization of Cellulose by Diazotrophic Strains of Paenibacillus polymyxa Isolated from Soil

Ewa Beata Górska1, Urszula Jankiewicz2, Jakub Dobrzyński1, Stefan Russel3, Stefan Pietkiewicz4, Hazem Kalaji4, Dariusz Gozdowski5 and Paweł Kowalczyk6*
1Department of Microbial Biology Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
2Department of Biochemistry, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
3Institute Technology and Life Sciences in Falenty, Hrabska 3, 05-090 Raszyn, Poland
4Department of Plant Physiology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
5Department of Experimental Statistics and Bioinformatics, Faculty of Agriculture and Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland
6Bionicum LTD, Chełmska 21, 00-724, Warsaw, Poland
Corresponding Author : Paweł Kowalczyk
Bionicum LTD, Chełmska 21
00-724, Warsaw, Poland
Tel: +48 22 840 66 99
E-mail: [email protected]
Received November 05, 2014; Accepted January 28, 2015; Published January 30, 2015
Citation: Górska EB, Jankiewicz U, Dobrzyński J, Russel S, Pietkiewicz S, et al. (2015) Degradation and Colonization of Cellulose by Diazotrophic Strains of Paenibacillus polymyxa Isolated from Soil. J Bioremed Biodeg 6:271. doi:10.4172/2155-6199.1000271
Copyright: © 2015 Górska EB, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Abstract

The bioconversion of cellulose to soluble sugars by diazotrophic bacteria is a very important for the environment, such as for the global stabilization and a sustainable human society. Two nitrogen-fixing microorganisms hydrolyzing cellulose were isolated from agricultural soil and identified as Paenibacillus polymyxa [the laboratory names EG2 and EG14] based on 16Sr RNA sequence. The genome of these bacteria was found to carry nif genes coding the individual components of the nitrogenase complex. Their nitrogen fixing ability was confirmed by studying nitrogenase activity in cultures of the studied bacteria in N-free medium supplemented with carboxymethylcellulose (CMC). The nitrogenase activity of P. polymyxa EG 2 was 2.9 nM C2H4?ml-1?h-1 whereas P. polymyxa EG 14 0.4nM C2H4?ml-1?h-1. The isolates in medium with filter paper synthesize following cellulolytic enzymes: carboxymethylcellulase (CMCase), FPase and Avicellase. Of the cellulolytic enzymes in the culture supernatants of the bacteria the most abundant was CMCase (P. polymyxa EG 2 103.4 mU, EG 14:96.1 mU) with far lower amounts of enzymes hydrolyzing crystalline Avicel cellulose or filter paper. In spite of these observations the better isolate in terms of synthesis of cellulases is P. polymyxa EG 14. Zymograms reflecting the main cellulase activities of the studied bacteria do not significantly differ from each other and present at least three major enzymatic activities with high molecular masses: one of about 200 kDa, another of about 220 kDa and a strong band of activity with mass of about 130 KDa. Observations of the bacterial cultures in medium with filter paper revealed the colonization of the substrate by single cells or aggregates of bacterial cells surrounded by slime. Scanning and transmission microscopy of the isolates revealed the presence of spherical structures resembling cellulosomes on the surface of the bacteria being characteristic for anaerobic bacteria of the genus Clostridium.

Keywords

Recommended Conferences

4th Annual Congress on Soil, Plant and Water Sciences

Barcelona, Spain

2nd World Congress on Biopolymers & Bioplastics

Webinar, Webinar
Share This Page
Top