alexa Detection of Rota Virus with the Help of Nanomaterial Based Field Effect Transistor (BIO-FET) | OMICS International| Abstract
ISSN: 2090-4967

Biosensors Journal
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
  • Research Article   
  • Biosens J 2017, Vol 6(2): 149
  • DOI: 10.4172/2090-4967.1000149

Detection of Rota Virus with the Help of Nanomaterial Based Field Effect Transistor (BIO-FET)

Mudita Pant1*, Kharkwal H1, Singh KP1 and Joshi HC2
1Department of Biophysics and Nanobiosensor Research Laboratory, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, India
2Department of Plant Physiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, India
*Corresponding Author : Mudita Pant, Department of Biophysics and Nanobiosensor Research Laboratory, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, India, Tel: + 0674 239 7970, Email: [email protected]

Received Date: Nov 29, 2017 / Accepted Date: Dec 13, 2017 / Published Date: Dec 20, 2017

Abstract

Biosensors are analytical devices composed of a recognition element of biological origin and a physiochemical transducer which converts the reaction into readable signals. The biosensors are providing their application in medical diagnosis and clinical analysis from last fifty years. In this work we have synthesized reduced graphene oxide, with this nano-film of RGO a FET- biosensor was developed and tested for the rapid detection of Rota-virus. It is a sensitive, specific and label free detection protocol. In the present methodology graphene; which is a single monomer unit of graphite was used and fabricated between the 3 μm apart micro-fabricated Au-electrode over hot plate at 70°C on a crystal surface by drop casting method. This thin film of graphene on FET crystal provide a better platform for attachment of antibodies through pyrene-NHS (Linker) complex. Pyrene- NHS is identified more suitable for antibody immobilization on the graphene film to be used in nano-immunosensor for the detection of specific antigens. The Rota-virus interation to its corresponding antibody were studied with respect to changes in the conductance in the graphene channel. This chemi-resistive FET transducer based 1D nanostructure have attracted much attention because of its rapid, sensitive, and cost effective point of care device for quantitative detection of Rota-virus so that gastroenteritis in infants can be detected early. The graphene nanofilm has been characterized by UV-Vis Spectroscopy, FT-IR, EDS, and SEM.

Keywords: Graphene oxide (GO); Reduced Graphene oxide (RGO); Field effect transistor (FET); Fourier transform infrared spectroscopy (FT-IR); Energy dispersive spectroscopy (EDS); Scanning electron microscopy (SEM)

Citation: Pant M, Kharkwal H, Singh KP, Joshi HC (2017) Detection of Rota Virus with the Help of Nanomaterial Based Field Effect Transistor (BIO-FET). Biosens J 6: 149. Doi: 10.4172/2090-4967.1000149

Copyright: ©2017 Pant M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Relevant Topics
Article Usage
  • Total views: 5270
  • [From(publication date): 0-2017 - Nov 20, 2019]
  • Breakdown by view type
  • HTML page views: 5120
  • PDF downloads: 150
Top