alexa Determination of Intracellular Concentrations of Nucleo
ISSN: 2329-9053

Journal of Molecular Pharmaceutics & Organic Process Research
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Review Article

Determination of Intracellular Concentrations of Nucleoside Analogues and their Phosphorylated Metabolites

Lingli Mu1*, Xingling Liu2, Sanwang Li2, Fang Tang2 and Peng Yu2
1Medical College, Hunan Normal University, Changsha 410006, China
2School of Pharmaceutical Sciences, Central South University, Changsha 410013, China
Corresponding Author : Lingli Mu
Medical College, Hunan Normal University
Changsha 410006, China
Tel: +867-318-265-0446
E-mail: [email protected]
Received February 24, 2014; Accepted April 28, 2014; Published April 30, 2014
Citation: Lingli Mu, Xingling Liu, Sanwang Li, Fang Tang, Peng Yu (2014) Determination of Intracellular Concentrations of Nucleoside Analogues and their Phosphorylated Metabolites. J Mol Pharm Org Process Res 2:112. doi: 10.4172/2329-9053.1000112
Copyright: © 2014 Lingli Mu, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Nucleoside analogues are broadly used in antiviral and anti-tumor therapy. The clinical response depends on the intracellular formation of the pharmacologically active mono-, di-, and tri-phosphates moiety. So it is not advisable to simply monitore plasma concentration without concerning the variation of concentration of active constituent in some particular effector cell apparently while this kind of drugs are applied to the clinical therapy. Therefore, determination of intracellular concentration of nucleoside analogues and their phosphorylated metabolites is of great significance. In this review, several methods including RP-HPLC, LC-MS/MS, radioimmunoassay and capillary electrophoresis (CE) for analysis of nucleoside analogues are discussed. Because of the complex biological matrices as well as the extremely low concentration of target analytes, cell lysis techniques and sample pretreatment methods such as protein precipitation (PP) and solid-phase extraction (SPE) are also discussed.

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