Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Determination of Intramolecular ¹³C Isotope Distribution of Pyruvate by Headspace Solid Phase Microextraction-Gas Chromatography-Pyrolysis-Gas Chromatography-Combustion- Isotope Ratio Mass Spectrometry (HS-SPMEGC-Py-GC-C-IRMS) Method

Tarin Nimmanwudipong¹, Naizhong Zhang³, Alexis Gilbert², Keita Yamada³ and Naohiro Yoshida^1,2,3

¹Department of Environmental Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan

²Earth-Life Science Institute (WPI-ELSI), Tokyo Institute of Technology, Meguro, Tokyo 152-8551, Japan

³Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan

*Corresponding Author:

Tarin Nimmanwudipong
Department of Environmental Science and Technology
Tokyo Institute of Technology, 4259 Nagatsuta
Midoriku, Yokohama 226-8502, Japan
Tel: +81459245517
Fax: +81459245517
E-mail: Nimmanwudipong.t.aa@m.titech.ac.jp

Received Date: Novmber 27, 2015; Accepted Date: December 21, 2015; Published Date: December 28, 2015

Citation: Nimmanwudipong T, Zhang N, Gilbert A, Yamada K, Yoshida N (2015) Determination of Intramolecular 13C Isotope Distribution of Pyruvate by Headspace Solid Phase Microextraction-Gas Chromatography-Pyrolysis-Gas Chromatography-Combustion- Isotope Ratio Mass Spectrometry (HS-SPMEGC-Py-GC-C-IRMS) Method. J Anal Bioanal Tech 7:293. doi:10.4172/2155-9872.1000293

Copyright: © 2015 Nimmanwudipong T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This paper presents the improvement of analytical methods for intramolecular carbon isotope compositions (δ13C) of pyruvate. Decarboxylated by H2O2, pyruvate yields acetic acid and CO2. Headspace solid phase micro-extractiongas chromatography-pyrolysis-gas chromatography-combustion-isotope ratio mass spectrometry (HS-SPME-GCPy- GC-C-IRMS) was used to measure the intramolecular δ13C values of acetic acid. δ13C value of CO2 can be later calculated using mass balance equation. The method’s consistency was confirmed by comparison of the δ13C value of CO2 from calculation to its direct measurement. Results of this study confirmed the method improvement because pyruvate 13C intramolecular distribution patterns were obtained. Two intramolecular 13C distribution patterns for commercial chemical reagents were found using this developed method. Intramolecular 13C distribution patterns for pyruvate were found for application in dietary supplements. Its origin was inferred. The method presented herein is expected to be a useful tool for categorization of pyruvate into different intramolecular 13C distribution patterns, which might indicate different production processes or raw materials.

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