Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Research Article

Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC

Yanbei Zhu*, Yuko Kitamaki, Megumi Kato, Tomoya Kinumi, Akiharu Hioki and Koichi Chiba

National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Umezono, Tsukuba, Ibaraki, Japan

*Corresponding Author:
Yanbei Zhu
National Metrology Institute of Japan
National Institute of Advanced Industrial Science and Technology
1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
Tel: 81-29-861-4130
E-mail: [email protected]

Received date: July 25, 2015; Accepted date: September 29, 2015; Published date: October 05, 2015

Citation: Zhu Y, Kitamaki Y, Kato M, Kinumi T, Hioki A, et al. (2015) Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC. J Anal Bioanal Tech 6:282. doi:10.4172/2155-9872.1000282

Copyright: © 2015 Zhu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


A method for determination of sulfur was investigated using an inductively coupled plasma tandem quadrupole mass spectrometer (ICP-QMS/QMS) with an octopole reaction/collision cell (ORC). It helped to achieve a lower background equivalent concentration (BEC) and a lower detection limit (DL) when 10% HNO3 with 10% ethanol was applied as the rinse solution. Sulfur measurement was carried out by shifting the measured mass from 32S+ to 32S16O+ by reaction with O2 gas. The introduction of H2 together with O2 as reaction gases provided a lower DL. The BEC and DL of 32S were 0.83 ng g-1 and 0.03 ng g-1, respectively. The validation of the present method was confirmed by determining sulfur in certified reference materials, NIST SRMs 2773, 2298, and 2299. The results of sulfur in amino-acids and protein indicate that determination of sulfur could be an effective technique for quantification of sulfur-containing amino acids and proteins.

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