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Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC

Yanbei Zhu*, Yuko Kitamaki, Megumi Kato, Tomoya Kinumi, Akiharu Hioki and Koichi Chiba

National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Umezono, Tsukuba, Ibaraki, Japan

*Corresponding Author:
Yanbei Zhu
National Metrology Institute of Japan
National Institute of Advanced Industrial Science and Technology
1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
Tel: 81-29-861-4130
E-mail: [email protected]

Received date: July 25, 2015; Accepted date: September 29, 2015; Published date: October 05, 2015

Citation: Zhu Y, Kitamaki Y, Kato M, Kinumi T, Hioki A, et al. (2015) Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC. J Anal Bioanal Tech 6:282. doi:10.4172/2155-9872.1000282

Copyright: © 2015 Zhu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A method for determination of sulfur was investigated using an inductively coupled plasma tandem quadrupole mass spectrometer (ICP-QMS/QMS) with an octopole reaction/collision cell (ORC). It helped to achieve a lower background equivalent concentration (BEC) and a lower detection limit (DL) when 10% HNO3 with 10% ethanol was applied as the rinse solution. Sulfur measurement was carried out by shifting the measured mass from 32S+ to 32S16O+ by reaction with O2 gas. The introduction of H2 together with O2 as reaction gases provided a lower DL. The BEC and DL of 32S were 0.83 ng g-1 and 0.03 ng g-1, respectively. The validation of the present method was confirmed by determining sulfur in certified reference materials, NIST SRMs 2773, 2298, and 2299. The results of sulfur in amino-acids and protein indicate that determination of sulfur could be an effective technique for quantification of sulfur-containing amino acids and proteins.

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Citations : 3366

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