Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC
Yanbei Zhu*, Yuko Kitamaki, Megumi Kato, Tomoya Kinumi, Akiharu Hioki and Koichi Chiba
National Metrology Institute of Japan, National Institute of Advanced Industrial Science and Technology, Umezono, Tsukuba, Ibaraki, Japan
- *Corresponding Author:
- Yanbei Zhu
National Metrology Institute of Japan
National Institute of Advanced Industrial Science and Technology
1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan
E-mail: [email protected]
Received date: July 25, 2015; Accepted date: September 29, 2015; Published date: October 05, 2015
Citation: Zhu Y, Kitamaki Y, Kato M, Kinumi T, Hioki A, et al. (2015) Determination of Sulfur in Bio-Samples by ICP-QMS/QMS with an ORC. J Anal Bioanal Tech 6:282. doi:10.4172/2155-9872.1000282
Copyright: © 2015 Zhu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A method for determination of sulfur was investigated using an inductively coupled plasma tandem quadrupole mass spectrometer (ICP-QMS/QMS) with an octopole reaction/collision cell (ORC). It helped to achieve a lower background equivalent concentration (BEC) and a lower detection limit (DL) when 10% HNO3 with 10% ethanol was applied as the rinse solution. Sulfur measurement was carried out by shifting the measured mass from 32S+ to 32S16O+ by reaction with O2 gas. The introduction of H2 together with O2 as reaction gases provided a lower DL. The BEC and DL of 32S were 0.83 ng g-1 and 0.03 ng g-1, respectively. The validation of the present method was confirmed by determining sulfur in certified reference materials, NIST SRMs 2773, 2298, and 2299. The results of sulfur in amino-acids and protein indicate that determination of sulfur could be an effective technique for quantification of sulfur-containing amino acids and proteins.