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Development and Validation of a Liquid Chromatography Method for the Analysis of Paromomycin Sulfate and its Impurities | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Development and Validation of a Liquid Chromatography Method for the Analysis of Paromomycin Sulfate and its Impurities

Ying Liu*, Shaorui Hou, Liping Wang and Shuxian Yang

Henan Provincial Institute for Food and Drug Control, Zhengzhou 450003, China

*Corresponding Author:
Ying Liu
Henan Provincial Institute for Food and Drug Control
Zhengzhou, China
Tel: 0086037163388265
Fax: 0086037163388265
E-mail: [email protected]

Received date: September 21, 2010; Accepted date: September 29, 2010; Published date: October 01, 2010

Citation: Liu Y, Hou S, Wang L, Yang S (2010) Development and Validation of a Liquid Chromatography Method for the Analysis of Paromomycin Sulfate and its Impurities. J Anal Bioanal Tech 1:102. doi: J Anal Bioanal Techniques 2010, 1:102

Copyright: © 2010 Liu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A rapid and simple method for the separation and quanti fi cation of paromomycin sulfate and its impurities by HPLC coupled with evaporative light scattering detection (ELSD) was developed. The chromatographic conditions included the use of a GRACE Alltima C 18 column (250mm×4.6mm, 5 μ m) maintained at 30°C and a mobile phase of 0.2 M tri fl uoroacetic acid water–acetonitrile (96:4, v/v) at a fl ow rate of 0.6 mL/min. The in fl uence of gas pressure and temperature of the drift tube in the detector on the detection response was also investigated. A system suitability test to check the quality of the separation was speci fi ed. The method showed good repeatability, linearity and robustness. It also enabled the simultaneous determination of the inorganic counter ions (sulfates).

Keywords

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Citations : 3732

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