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Development and Validation of an HPLC Method for Quantifying Dapiprazole in Bulk Preparations | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Development and Validation of an HPLC Method for Quantifying Dapiprazole in Bulk Preparations

Jaya Prasanthi K and Syama Sundar B*

Department of Chemistry, Acharya Nagarjuna University, Guntur, India

*Corresponding Author:
B. Syama Sundar
Department of Chemistry
Acharya Nagarjuna University
Nagarjuna Nagar, Guntur-522510, India
E-mail:
[email protected]

Received date: July 16, 2012; Accepted date: August 24, 2012; Published date: August 30, 2012

Citation: Jaya Prasanthi K, Syama Sundar B (2012) Development and Validation of an HPLC Method for Quantifying Dapiprazole in Bulk Preparations. J Anal Bioanal Tech 3:143. doi: 10.4172/2155-9872.1000143

Copyright: © 2012 Jaya Prasanthi K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple, precise, rapid and accurate Reverse phase HPLC method was developed for the estimation of Dapiprazole in bulk form. A Thermo hypersil C-18 column (250 mm×4.6 mm 5μm) with mobile phase consisting of mixture of methanol: acetonitrile: and ortho-phosphoric acid in the ratio of 89: 9: 1 (v/v) at pH 5.8 adjusted with ortho-phosphoric acid. The flow rate was 0.8 mL/min and the effluents were monitored at 243 nm. The retention time was 3.725 min. The detector response was linear in the concentration of 20-120 μg/mL. The respective linear regression equation being y=1956.4x+3409. The limit of detection and limit of quantification was 0.5 μg/mL and 1.6 μg/mL respectively. The percentage assay of Dapiprazole was 99.94%. This method has been validated and shown to be specific, sensitive, precise, linear, accurate, rugged, robust and fast. Hence, this method can be useful for the routine determination of Dapiprazole in bulk drug and in its pharmaceutical dosage form.

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