alexa Development and Validation of Spectrophotometric and HPLC Method for the Simultaneous Estimation of Salbutamol Sulphate and Prednisolone in Tablet Dosage Form
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Development and Validation of Spectrophotometric and HPLC Method for the Simultaneous Estimation of Salbutamol Sulphate and Prednisolone in Tablet Dosage Form

Sohan S. Chitlange*, Kaushalendra K. Chaturvedi and Sagar B. Wankhede

Padmashri Dr. D.Y. Patil Institute of Pharmaceutical sciences and Research, Sant Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, India

*Corresponding Author:
Dr. Sohan S. Chitlange
Padmashri Dr. D.Y. Patil Institute of Pharmaceutical sciences and Research, Sant Tukaram Nagar
Pimpri, Pune-411018, Maharashtra, India
Tel: +91-9922904305
E-mail: sohanchitlange@rediffmail.com

Received date: December 27, 2010; Accepted date: February 11, 2011; Published date: March 02, 2011

Citation: Chitlange SS, Chaturvedi KK, Wankhede SB (2011) Development and Validation of Spectrophotometric and HPLC Method for the Simultaneous Estimation of Salbutamol Sulphate and Prednisolone in Tablet Dosage Form. J Anal Bioanal Tech 2:117. doi: 10.4172/2155-9872.1000117

Copyright: © 2011 Chitlange SS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Simple, accurate, and reproducible UV spectrophotometric and HPLC method for simultaneous estimation of salbutamol (SAL) and prednisolone (PRE) was developed in the present work. The first developed method was Simultaneous equation method, wavelength selected are 227 nm for salbutamol and 244 nm for prednisolone respectively. Linearity was observed in concentration range of 6-20μg/ml for salbutamol as well as for prednisolone. Second developed method was RP-HPLC method using Thermo C18 column (4.6 mm i.d × 250 mm) and acetonitrile: 0.025M potassium dihydrogen orthophosphate buffer (pH adjusted to 3.5 with orthophosphoric acid) in the ratio of 30:70% v/v as mobile phase. For HPLC method, linearity was observed in the concentration range of 20-100μg/ml for salbutamol as well as for prednisolone and drugs was subjected to oxidation, hydrolysis, and heat to apply stress condition for degradation studies. Results of analysis were validated stastically and by recovery studies.

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