Development of Molecular Identification of Nitrifying Bacteria in Water Bodies of East Kolkata Wetland, West Bengal | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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Research Article

Development of Molecular Identification of Nitrifying Bacteria in Water Bodies of East Kolkata Wetland, West Bengal

Mousumi Saha1*, Agniswar Sarkar2 and Bidyut Bandhophadhyay1
1Department of Biotechnology, Vidyasagar University, West Bengal, India
2Department of Biotechnology (Recognized by DBT, Govt. of India), The University of Burdwan, Burdwan, West Bengal, India
Corresponding Author : Mousumi Saha
Department of Biotechnology
Vidyasagar University
West Bengal, India
Tel: +91 9830736189
E-mail: [email protected]
Received: September 17, 2013; Accepted: October 30, 2013; Published: November 07, 2013
Citation: Saha M, Sarkar A, Bandhophadhyay B (2013) Development of Molecular Identification of Nitrifying Bacteria in Water Bodies of East Kolkata Wetland, West Bengal. J Bioremed Biodeg 4:211. doi:10.4172/2155-6199.1000211
Copyright: © 2013 Saha M, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Nitrifying bacteria plays a major role in converting the waste water to valuable renewable resources for the society. Ammonia is the toxic excretory product of most aquatic organisms and nitrite formed by the oxidation of ammonia is also toxic. Ammonia oxidizing bacteria converts ammonia to nitrite and Nitrite oxidizing bacteria convert nitrite to nitrate. Among them, Nitrosomonas sp. and Nitrobacter sp. has been the most widely studied organisms. An advanced molecular technique made it possible to explore the nitrifying bacteria in the environment and to enhance our knowledge of its functioning. In view of this it would be of prime importance for rapid detection of these strains/ isolates using molecular techniques. The present study was undertaken to develop and validate 16S rDNA sequence analysis of Nitrosomonas sp. and Nitrobacter sp. collected from different bheries in East Kolkata Wetland, West Bengal.


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