JBR Journal of Interdisciplinary Medicine and Dental Science
Open Access

Research Article

Effect of Fluoride Application Combined with CO₂ Laser Irradiation on the Demineralization/Remineralization of Root Surfaces

Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata City, Niigata 951-8580, Japan

*Corresponding Author:

Koichi Shinkai
Department of Operative Dentistry
The Nippon Dental University School of Life Dentistry at Niigata
1-8 Hamaura-cho, Chuoku, Niigata City
Niigata 951-8580, Japan
Tel: +81-25-267-1500
Fax: +81-25-265-7259
E-mail: shinkaik@ngt.ndu.ac.jp

Received date: July 15, 2017; Accepted date: July 20, 2017; Published date: July 27, 2017

Citation: Shinkai K, Kawashima S, Suzuki M, Suzuki S (2017) Effect of Fluoride Application Combined with CO₂ Laser Irradiation on the Demineralization/Remineralization of Root Surfaces. J Interdiscipl Med Dent Sci 5: 213. doi: 10.4172/2376-032X.1000213

Copyright: © 2017 Shinkai K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Objectives: The purpose of this study was to evaluate the effect of CO2 laser irradiation at different energy densities followed by fluoride application at various concentrations on the demineralization/remineralization of root surfaces. Materials and methods: The roots of 30 extracted human premolars were cleaned, and all surfaces except for a window (2×3 mm) on the proximal surface were coated with an acid-resistant varnish. The root specimens were divided into two groups according to laser energy densities (17 and 25 J/cm2 ). Each group was further divided into three subgroups according to fluoride concentration (0.05,0.2 and 2.0% NaF). After each treatment was performed on the uncoated surface windows, the specimens were subjected to a pH-cycling test in which the roots were subjected to a cycle of demineralization (pH 4.7 for 18 hours) and remineralization (pH 7.0 for 6 hours) for 2 days. From each window, 4 sections (100 μm thick) were obtained. The lesion depth (LD) within the sections was measured with polarized light microscopy. The data were statistically analyzed with two-way ANOVA and Tukey’s post hoc test. Results: The results of a two-way ANOVA showed that both laser energy and fluoride concentration had significant effects on the LD (p

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