Effect of Heavy Metal Uptake by E. coli and Bacillus sps | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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Research Article

Effect of Heavy Metal Uptake by E. coli and Bacillus sps

Vijayadeep C1* and Sastry PS2
1Research Scholar, Department of Microbiology, Maharani Lakshmi Ammanni College for Women, Science PO, Malleswaram, Bangalore, Bangalore university, India
2HOD, Department of Botany & Microbiology, Vijaya College, Bangalore, India
Corresponding Author : Vijayadeep C
Research Scholar, Department of Microbiology
Maharani Lakshmi Ammanni College for Women
Science PO, Malleswaram, Bangalore
Bangalore University, India
Tel: 9886755403
E-mail: [email protected]
Received May 19, 2014; Accepted July 14, 2014; Published July 18, 2014
Citation: Vijayadeep C, Sastry PS (2014) Effect of Heavy Metal Uptake by E. coli and Bacillus sps. J Bioremed Biodeg 5:238. doi:10.4172/2155-6199.1000238
Copyright: © 2014 Vijayadeep C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Over the past century, unrestricted mining, extensive industrialization, modern agricultural practices and faulty waste disposal methods have resulted in the release of unprecedented levels of toxic heavy metals like Cd, Hg, Ag, Sn, Pb, Cu, Co, Mn, Zn, etc into the environment. Many metals are essential for microbial growth in less concentration, yet are toxic in higher concentrations. Biosorption is an attractive alternative approach which involves the binding or adsorption of heavy metals to living or dead cells. Many microbes have the ability to selectively accumulate metals.

The present study is intended to analyze the uptake systems of Bacillus and E. coli against different conc. of heavy metals like Zn, Cu, Cd, and Hg in their salt form incorporated into nutrient broth medium observed over a regular interval of time. Analysis was based on how much of the metal from the original conc. Used was left behind in the media after the rest being up taken by the organism. This was done using AAS which was indirectly the representation of percent uptake of heavy metal by the respective organism.

The study showed that Gram –ve organisms like E. coli exhibited more resistance to metals like Zn, Cu and Hg in relative comparison with Gram +ve organisms like Bacillus. Bacillus sps was less sensitive to effect of Cd than in E. coli.


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