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Effect of Lewy Bodies on Mitochondrial DNA Copy Numbers and Deletion Burden in Parkinson's Disease Substantia nigra Neurons | OMICS International | Abstract
ISSN: 2161-0460

Journal of Alzheimers Disease & Parkinsonism
Open Access

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Research Article

Effect of Lewy Bodies on Mitochondrial DNA Copy Numbers and Deletion Burden in Parkinson's Disease Substantia nigra Neurons

Knarik Arkun1*, Ann C Rice2,3 and James P Bennett2-5

1Department of Pathology, Virginia Commonwealth University, Richmond VA, 23298

2Parkinson’s and Movement Disorder Center, Virginia Commonwealth University, Richmond VA, USA

3Department of Neurology, Virginia Commonwealth University, Richmond VA, 23298

4Psychiatry, Virginia Commonwealth University, Richmond VA, 23298

5Physiology/Biophysics, Virginia Commonwealth University, Richmond VA, 23298

Corresponding Author:
Knarik Arkun
Department of Pathology
Virginia Commonwealth University
Richmond VA, 23298, USA
Tel: 617-636-5031
Fax: 617-636-8302
E-mail: [email protected]

Received date: November 7, 2014; Accepted date: December 31, 2014; Published date: January 7, 2015

Citation: Arkun K, Rice AC, Bennett JP (2015) Effect of Lewy Bodies on Mitochondrial DNA Copy Numbers and Deletion Burden in Parkinson’s Disease Substantia nigra Neurons. J Alzheimers Dis Parkinsonism 5:175. doi:10.4172/2161-0460.1000175

Copyright: © 2015 Arkun K et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objective: Our objective was to evaluate mitochondrial DNA (mtDNA) gene copy numbers in substantia nigra neurons from post-mortem Parkinson’s Disease cases and determine if the presence of Lewy bodies (LBs) altered mtDNA copy numbers or changed apparent deletion of mtDNAs. Methods: We used laser capture micro dissection to isolate neuromelanin-containing cells with or without Lewy bodies from 6 Parkinson’s Disease cases. Anti-alpha-synuclein immunohistochemistry was used to identify Lewy bodies in flash frozen tissue. Cells were collected in triplicate groups of 15 cells (LB (+) or LB (-)) from each case, and DNA was extracted with proteinase K-containing buffer. mtDNA copy number was determined by quantitative PCR for 4 genes distributed around the mitochondrial genome (12S rRNA; ND2; CO3; ND4) and normalized to a copy number standard curve of human mtDNA run on the same plate. Results: We found an average ~4-fold higher mtDNA copy numbers in LB (+) neurons compared to LB (-) neurons from the same cases, but we observed substantial heterogeneity. ND4 appeared to be deleted in all cases. ND2 appeared to be deleted in one case as was CO3 in two cases. Conclusion: Our studies indicate that in most cases of synucleinopathy, LB presence does not reduce, and appears to increase, mtDNA copy numbers in PD nigral neuromelanin-containing neurons.

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