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Hindi Research Article
Effects of Growth Regulators and Sucrose on in vitro Nodal Segments and Shoot Tip Culture of Jojoba (Simmondsia chinensis (Link) Genotypes
| Amr SM Mohamed*, Magdi AA Mousa and Ahmed AS Bakhashwain | |
| Department of Arid land Agriculture, Faculty of Meteorology, Environment and Arid land Agriculture, King Abdulaziz University, Saudi Arabia | |
| Corresponding Author : | Amr SM Mohamed Department of Arid land Agriculture Faculty of Meteorology Environment and Arid land Agriculture King Abdulaziz University, Saudi Arabia E-mail: amrsalahw@yahoo.com |
| Received: July 20, 2013; Accepted: August 27, 2013; Published: August 29, 2013 | |
| Citation: Mohamed ASM, Mousa MAA, Bakhashwain AAS (2013) Effects of Growth Regulators and Sucrose on in vitro Nodal Segments and Shoot Tip Culture of Jojoba (Simmondsia chinensis (Link) Genotypes. J Bioremed Biodeg 4:202. doi:10.4172/2155-6199.1000202 | |
| Copyright: © 2013 Mohamed ASM, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
The responses of four jojoba genotypes to in vitro nodal segments and shoot tip culture under different growth regulators combinations and sucrose concentrations were investigated. All experiments were laid out in Completely Randomized Blocks (CR) design using 5 replicates. The results revealed that the jojoba genotype ‘Al-Maddenah’ conveyed the highest response to nodal segments, with MS culture medium supplemented with BAP (3 mg) alone and BAP (3 mg), in combination with IAA (7 mg). Modifying sucrose concentration in the culture medium (20 g, 40 g, 50 g and 60 g) enhanced shoot formation of nodal segments of the genotype ‘Hada Al-Sham”. Concerning shoot tip culture, explants of the genotype ‘Hada Al-Sham’ were cultured on MS+BAP (5 mg)+IAA (5 mg) produced the highest percentages of bud sprouting and shoot formation. Increasing sucrose concentration from 20 g to 60 allowed the genotype ‘Hail-B’ to produced the highest percentage of bud sprouting and shoot formation.
